ArticleProceedings of the National Academy of Sciences of the United States of America1999
Transcriptional activation by artificial recruitment in mammalian cells.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 42 citations in OpenAlex.
- Article
- Article
- Transition step during assembly of HIV Tat:P-TEFb transcription complexes and transfer to TAR RNA.Molecular and cellular biology · 2012Article
- Transcriptional activators and activation mechanisms.Protein & cell · 2011Review
- Regulating RNA polymerase pausing and transcription elongation in embryonic stem cells.Genes & development · 2011Article
- The transition from transcriptional initiation to elongation.Current opinion in genetics & development · 2008Review
- TFIIB and the regulation of transcription by RNA polymerase II.Chromosoma · 2007Review
- Specific TATAA and bZIP requirements suggest that HTLV-I Tax has transcriptional activity subsequent to the assembly of an initiation complex.Retrovirology · 2004Article
- Antithetic effects of MBD2a on gene regulation.Molecular and cellular biology · 2003Article
- Protein-protein interaction map for yeast TFIID.Nucleic acids research · 2003Article
- The coactivator dTAF(II)110/hTAF(II)135 is sufficient to recruit a polymerase complex and activate basal transcription mediated by CREB.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- Transcription activation by targeted recruitment of the RNA polymerase II CTD phosphatase FCP1.Nucleic acids research · 2001Article
- Dual roles of RNA helicase A in CREB-dependent transcription.Molecular and cellular biology · 2001Article
- Transcriptional adaptor and histone acetyltransferase proteins in Arabidopsis and their interactions with CBF1, a transcriptional activator involved in cold-regulated gene expression.Nucleic acids research · 2001Article
- Artificially recruited TATA-binding protein fails to remodel chromatin and does not activate three promoters that require chromatin remodeling.Molecular and cellular biology · 2000Article
- A role of transcriptional activators as antirepressors for the autoinhibitory activity of TATA box binding of transcription factor IID.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Artificial recruitment of TFIID, but not RNA polymerase II holoenzyme, activates transcription in mammalian cells.Molecular and cellular biology · 2000Article
- An artificial transcriptional activating region with unusual properties.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Chemical approaches to control gene expression.Gene expression · 2000Review
- The N-terminal domain of human TAFII68 displays transactivation and oncogenic properties.Oncogene · 1999Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
We show that the typical "nonclassical" activator, which comprises a fusion protein bearing a component of the transcriptional machinery fused to a DNA-binding domain, activates transcription in mammalian cells only weakly when tested with an array of promoters. However, as found in analogous "artificial recruitment" experiments performed in yeast, these activators work synergistically with "classical" activators. The effect of the classical activator in such experiments requires that it be tethered to DNA, a requirement that cannot be overcome by expression of that classical activator at high levels. The effect of the one nonclassical activator that does elicit significant levels of transcription when working alone (i.e., that bearing TATA box-binding protein) is strongly influenced by promoter architecture. The results, consistent with those of analogous experiments in yeast [see the accompanying paper: Gaudreau, L., Keaveney, M., Nevado, J., Zaman, Z., Bryant, G. O., Struhl, K. & Ptashne, M. (1999) Proc. Natl. Acad. Sci. USA 96, 2668-2673], suggest that classical activators, presumably by virtue of their abilities to interact with multiple targets, have a functional flexibility that nonclassical activators lack.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.