ArticleProceedings of the National Academy of Sciences of the United States of America1999
Transcriptional activation by artificial recruitment in yeast is influenced by promoter architecture and downstream sequences.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 58 citations in OpenAlex.
- Revisiting the model for coactivator recruitment: Med15 can select its target sites independent of promoter-bound transcription factors.Nucleic acids research · 2024Article
- An overview and future prospects of recombinant protein production in Bacillus subtilis.Applied microbiology and biotechnology · 2021Review
- Article
- Mediator Undergoes a Compositional Change during Transcriptional Activation.Molecular cell · 2016Article
- Using synthetic biology to make cells tomorrow's test tubes.Integrative biology : quantitative biosciences from nano to macro · 2016Review
- Fungal mediator tail subunits contain classical transcriptional activation domains.Molecular and cellular biology · 2015Article
- Activation of silenced cytokine gene promoters by the synergistic effect of TBP-TALE and VP64-TALE activators.PloS one · 2014Article
- YeTFaSCo: a database of evaluated yeast transcription factor sequence specificities.Nucleic acids research · 2012Article
- Review
- Phosphorylation of the Gal4 DNA-binding domain is essential for activator mono-ubiquitylation and efficient promoter occupancy.Molecular bioSystems · 2008Article
- Targeting of Swi/Snf to the yeast GAL1 UAS G requires the Mediator, TAF IIs, and RNA polymerase II.The EMBO journal · 2004Article
- Mutations in the histone fold domain of the TAF12 gene show synthetic lethality with the TAF1 gene lacking the TAF N-terminal domain (TAND) by different mechanisms from those in the SPT15 gene encoding the TATA box-binding protein (TBP).Nucleic acids research · 2003Article
- Protein-protein interaction map for yeast TFIID.Nucleic acids research · 2003Article
- The role of TFIIB-RNA polymerase II interaction in start site selection in yeast cells.Nucleic acids research · 2002Article
- A target essential for the activity of a nonacidic yeast transcriptional activator.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- The yeast protein Xtc1 functions as a direct transcriptional repressor.Nucleic acids research · 2002Article
- H2A.Z is required for global chromatin integrity and for recruitment of RNA polymerase II under specific conditions.Molecular and cellular biology · 2001Article
- Transcriptional adaptor and histone acetyltransferase proteins in Arabidopsis and their interactions with CBF1, a transcriptional activator involved in cold-regulated gene expression.Nucleic acids research · 2001Article
- Artificially recruited TATA-binding protein fails to remodel chromatin and does not activate three promoters that require chromatin remodeling.Molecular and cellular biology · 2000Article
- A role of transcriptional activators as antirepressors for the autoinhibitory activity of TATA box binding of transcription factor IID.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
The idea that recruitment of the transcriptional machinery to a promoter suffices for gene activation is based partly on the results of "artificial recruitment" experiments performed in vivo. Artificial recruitment can be effected by a "nonclassical" activator comprising a DNA-binding domain fused to a component of the transcriptional machinery. Here we show that activation by artificial recruitment in yeast can be sensitive to any of three factors: position of the activator-binding elements, sequence of the promoter, and coding sequences downstream of the promoter. In contrast, classical activators worked efficiently at all promoters tested. In all cases the "artificial recruitment" fusions synergized well with classical activators. A classical activator evidently differs from a nonclassical activator in that the former can touch multiple sites on the transcriptional machinery, and we propose that that difference accounts for the broader spectrum of activity of the typical classical activator. A similar conclusion is reached from studies in mammalian cells in the accompanying paper [Nevado, J., Gaudreau, L., Adam, M. & Ptashne, M. (1999) Proc. Natl. Acad. Sci. USA 96, 2674-2677].
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.