ArticleProceedings of the National Academy of Sciences of the United States of America1999
The fission yeast homologue of Orc4p binds to replication origin DNA via multiple AT-hooks.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 112 papers.
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112 citing papers in PubMed, 238 citations in OpenAlex.
- Binding to the Other Side: The AT-Hook DNA-Binding Domain Allows Nuclear Factors to Exploit the DNA Minor Groove.International journal of molecular sciences · 2024Review
- The origin recognition complex requires chromatin tethering by a hypervariable intrinsically disordered region that is functionally conserved from sponge to man.Nucleic acids research · 2024Article
- The genetic landscape of origins of replication in P. falciparum.Nucleic acids research · 2024Article
- The Origin Recognition Complex: From Origin Selection to Replication Licensing in Yeast and Humans.Biology · 2023Review
- Origins of DNA replication in eukaryotes.Molecular cell · 2023Review
- DNA replication timing: Biochemical mechanisms and biological significance.BioEssays : news and reviews in molecular, cellular and developmental biology · 2022Article
- Characterization of Episomal Replication of Bovine Papillomavirus Type 1 DNA in Long-Term Virion-Infected Saccharomyces Cerevisiae Culture.Virologica Sinica · 2021Article
- Chromosomal Mcm2-7 distribution and the genome replication program in species from yeast to humans.PLoS genetics · 2021Article
- Orc4 spatiotemporally stabilizes centromeric chromatin.Genome research · 2021Article
- Humanizing the yeast origin recognition complex.Nature communications · 2021Article
- Evolution of DNA replication origin specification and gene silencing mechanisms.Nature communications · 2020Article
- Structural mechanism for replication origin binding and remodeling by a metazoan origin recognition complex and its co-loader Cdc6.Nature communications · 2020Article
- Bioinformatical dissection of fission yeast DNA replication origins.Open biology · 2020Article
- TASks for subtelomeres: when nucleosome loss and genome instability are favored.Current genetics · 2019Review
- A new class of disordered elements controls DNA replication through initiator self-assembly.eLife · 2019Article
- Origins of DNA replication.PLoS genetics · 2019Review
- Dynamics of DNA replication in a eukaryotic cell.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Mathematical description of eukaryotic chromosome replication.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Autonomously Replicating Linear Plasmids That Facilitate the Analysis of Replication Origin Function inmSphere · 2019Article
- Shelterin and subtelomeric DNA sequences control nucleosome maintenance and genome stability.EMBO reports · 2019Article
52 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The origin recognition complex (ORC) was originally identified in the yeast Saccharomyces cerevisiae as a protein that specifically binds to origins of DNA replication. Although ORC appears to play an essential role in the initiation of DNA replication in the cells of all eukaryotes, its interactions with DNA have not been defined in species other than budding yeast. We have characterized a Schizosaccharomyces pombe homologue of the ORC subunit, Orc4p. The homologue (Orp4p) consists of two distinct functional domains. The C-terminal domain shows strong sequence similarity to human, frog, and yeast Orc4 proteins, including conserved ATP-binding motifs. The N-terminal domain contains nine copies of the AT-hook motif found in a number of DNA-binding proteins, including the members of the HMG-I(Y) family of chromatin proteins. AT-hook motifs are known from biochemical and structural studies to mediate binding to the minor groove of AT-tracts in DNA. Orp4p is essential for viability of Sc. pombe and is expressed throughout the cell cycle. The Orp4 protein (and its isolated N-terminal domain) binds to the Sc. pombe replication origin, ars1. The DNA binding properties of Orp4p provide a plausible explanation for the characteristic features of Sc. pombe origins of replication, which differ significantly from those of Sa. cerevisiae.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.