ArticleProceedings of the National Academy of Sciences of the United States of America1999
Structural characteristics of supramolecular assemblies formed by guanidinium-cholesterol reagents for gene transfection.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 95 citations in OpenAlex.
- Lipids and Lipid Derivatives for RNA Delivery.Chemical reviews · 2021Review
- X-ray induced photodynamic therapy (PDT) with a mitochondria-targeted liposome delivery system.Journal of nanobiotechnology · 2020Article
- Tetrafunctional Block Copolymers Promote Lung Gene Transfer in Newborn Piglets.Molecular therapy. Nucleic acids · 2019Article
- Dual Functional LipoMET Mediates Envelope-type Nanoparticles to Combinational Oncogene Silencing and Tumor Growth Inhibition.Molecular therapy : the journal of the American Society of Gene Therapy · 2017Article
- A Simplified Direct Lipid Mixing Lipoplex Preparation: Comparison of Liposomal-, Dimethylsulfoxide-, and Ethanol-Based Methods.Scientific reports · 2016Article
- Fluorescent mimics of cholesterol that rapidly bind surfaces of living mammalian cells.Chemical communications (Cambridge, England) · 2015Article
- Applications of synchrotron-based spectroscopic techniques in studying nucleic acids and nucleic acid-functionalized nanomaterials.Advanced materials (Deerfield Beach, Fla.) · 2014Review
- Amphiphilic block copolymers enhance the cellular uptake of DNA molecules through a facilitated plasma membrane transport.Nucleic acids research · 2011Article
- Probing the in vitro mechanism of action of cationic lipid/DNA lipoplexes at a nanometric scale.Nucleic acids research · 2011Article
- Review
- Practical synthesis of 3beta-amino-5-cholestene and related 3beta-halides involving i-steroid and retro-i-steroid rearrangements.Organic letters · 2009Article
- Self-assembled lamellar complexes of siRNA with lipidic aminoglycoside derivatives promote efficient siRNA delivery and interference.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Physicochemical properties affecting lipofection potency of a new series of 1,2-dialkoylamidopropane-based cationic lipids.Colloids and surfaces. B, Biointerfaces · 2007Article
- Structural investigations of DNA-polycation complexes.The European physical journal. E, Soft matter · 2005Article
- Negatively charged self-assembling DNA/poloxamine nanospheres for in vivo gene transfer.Nucleic acids research · 2004Article
- Combined suicide gene therapy for pancreatic peritoneal carcinomatosis using BGTC liposomes.Cancer gene therapy · 2004Article
- Lipoplex thermodynamics: determination of DNA-cationic lipoid interaction energies.Biophysical journal · 2003Article
- Reducible cationic lipids for gene transfer.The Biochemical journal · 2001Article
- The phase behavior of cationic lipid-DNA complexes.Biophysical journal · 2000Article
- Vectors for gene therapy of cardiovascular disease.Current cardiology reports · 2000Review
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Authors and funding
12 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have recently discovered that cationic cholesterol derivatives characterized by guanidinium polar headgroups are very efficient for gene transfection in vitro and in vivo. In spite of being based on some rationale at the molecular level, the development of these new synthetic vectors was nevertheless empirical. Indeed, the factors and processes underlying cationic lipid-mediated gene transfer are still poorly understood. Thus, to get a better insight into the mechanisms involved, we have examined the supramolecular structure of lipid/DNA aggregates obtained when using reagent bis(guanidinium)-tren-cholesterol (BGTC), either alone or as a liposomal formulation with the neutral phospholipid dioleoyl phosphatidylethanolamine (DOPE). We here report the results of cryotransmission electron microscopy studies and small-angle x-ray scattering experiments, indicating the presence of multilamellar domains with a regular spacing of 70 A and 68 A in BGTC/DOPE-DNA and BGTC-DNA aggregates, respectively. In addition, DNA lipoplexes with similar lamellar patterns were detected inside transfected HeLa cells by conventional transmission electron microscopy. These results suggest that DNA condensation by multivalent guanidinium-cholesterol cationic lipids involves the formation of highly ordered multilamellar domains, the DNA molecules being intercalated between the lipid bilayers. These results also invite further investigation of the intracellular fate of the internalized lipid/DNA structures during their trafficking toward the cell nucleus. The identification of the basic features of active complexes should indeed help in the design of improved guanidinium-based vectors.
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