ArticleGenome research1999
An efficient DNA sequencing strategy based on the bacteriophage mu in vitro DNA transposition reaction.
Article in Genome research, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Light-switchable transcription factors obtained by direct screening in mammalian cells.Nature communications · 2023Article
- A primer to directed evolution: current methodologies and future directions.RSC chemical biology · 2023Review
- Mu transpososome activity-profiling yields hyperactive MuA variants for highly efficient genetic and genome engineering.Nucleic acids research · 2018Article
- Development of novel metabolite-responsive transcription factors via transposon-mediated protein fusion.Protein engineering, design & selection : PEDS · 2018Article
- Rapid construction of metabolite biosensors using domain-insertion profiling.Nature communications · 2016Article
- MuA-mediated in vitro cloning of circular DNA: transpositional autointegration and the effect of MuB.Molecular genetics and genomics : MGG · 2016Article
- An assay to monitor the activity of DNA transposition complexes yields a general quality control measure for transpositional recombination reactions.Mobile genetic elements · 2014Article
- Article
- Genome sequence of Lactobacillus amylovorus GRL1118, isolated from pig ileum.Journal of bacteriology · 2011Article
- Transposition-based method for the rapid generation of gene-targeting vectors to produce Cre/Flp-modifiable conditional knock-out mice.PloS one · 2009Article
- Bacteriophage Mu integration in yeast and mammalian genomes.Nucleic acids research · 2008Article
- High-precision mapping of protein protein interfaces: an integrated genetic strategy combining en masse mutagenesis and DNA-level parallel analysis on a yeast two-hybrid platform.Nucleic acids research · 2007Article
- SNP discovery by mismatch-targeting of Mu transposition.Nucleic acids research · 2007Article
- Genome characterization of lipid-containing marine bacteriophage PM2 by transposon insertion mutagenesis.Journal of virology · 2006Article
- A gene truncation strategy generating N- and C-terminal deletion variants of proteins for functional studies: mapping of the Sec1p binding domain in yeast Mso1p by a Mu in vitro transposition-based approach.Nucleic acids research · 2005Article
- Identification of viral genes essential for replication of murine gamma-herpesvirus 68 using signature-tagged mutagenesis.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- A direct transposon insertion tool for modification and functional analysis of viral genomes.Journal of virology · 2003Article
- Systematic sequencing of cDNA clones using the transposon Tn5.Nucleic acids research · 2002Article
- Functional genomics on potato virus A: virus genome-wide map of sites essential for virus propagation.Genome research · 2002Article
- Efficient insertion mutagenesis strategy for bacterial genomes involving electroporation of in vitro-assembled DNA transposition complexes of bacteriophage mu.Applied and environmental microbiology · 2002Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A highly efficient DNA sequencing strategy was developed on the basis of the bacteriophage Mu in vitro DNA transposition reaction. In the reaction, an artificial transposon with a chloramphenicol acetyltransferase (cat) gene as a selectable marker integrated into the target plasmid DNA containing a 10.3-kb mouse genomic insert to be sequenced. Bacterial clones carrying plasmids with the transposon insertions in different positions were produced by transforming transposition reaction products into Escherichia coli cells that were then selected on appropriate selection plates. Plasmids from individual clones were isolated and used as templates for DNA sequencing, each with two primers specific for the transposon sequence but reading the sequence into opposite directions, thus creating a minicontig. By combining the information from overlapping minicontigs, the sequence of the entire 10,288-bp region of mouse genome including six exons of mouse Kcc2 gene was obtained. The results indicated that the described methodology is extremely well suited for DNA sequencing projects in which considerable sequence information is on demand. In addition, massive DNA sequencing projects, including those of full genomes, are expected to benefit substantially from the Mu strategy.
Indexed as
Identifiers
10077537PMC310728What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.