ArticleGenome research1999
Postsynaptic alpha-neurotoxin gene of the spitting cobra, Naja naja sputatrix: structure, organization, and phylogenetic analysis.
Article in Genome research, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers, 1 of them a synthesis that pooled it.
What it found
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The trial behind it
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Who cites it
13 citing papers in PubMed, 1 synthesis or guideline pooled it, 57 citations in OpenAlex.
- Pooled it
- Variations in "Functional Site" Residues and Classification of Three-Finger Neurotoxins in Snake Venoms.Toxins · 2025Review
- A Review of Rattlesnake Venoms.Toxins · 2023Review
- Dynamic genetic differentiation drives the widespread structural and functional convergent evolution of snake venom proteinaceous toxins.BMC biology · 2022Article
- Toxinology provides multidirectional and multidimensional opportunities: A personal perspective.Toxicon: X · 2020Article
- Article
- Orphan Three-Finger Toxins Bind at Tissue Factor-Factor VIIa Interface to Inhibit Factor X Activation: Identification of Functional Site by Docking.TH open : companion journal to thrombosis and haemostasis · 2018Article
- Prediction of presynaptic and postsynaptic neurotoxins by combining various Chou's pseudo components.Scientific reports · 2017Article
- Accelerated exchange of exon segments in Viperid three-finger toxin genes (Sistrurus catenatus edwardsii; Desert Massasauga).BMC evolutionary biology · 2008Article
- Molecular evolution and phylogeny of elapid snake venom three-finger toxins.Journal of molecular evolution · 2003Article
- Characterization and gene organization of Taiwan banded krait (Bungarus multicinctus) gamma-bungarotoxin.Journal of protein chemistry · 2002Article
- Cloning and characterization of the pseudonajatoxin b precursor.The Biochemical journal · 2001Article
- Real-time detection of gene promoter activity: quantitation of toxin gene transcription.Nucleic acids research · 2001Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The venom of the spitting cobra, Naja naja sputatrix contains highly potent alpha-neurotoxins (NTXs) in addition to phospholipase A2 (PLA2) and cardiotoxin (CTX). In this study, we report the complete characterization of three genes that are responsible for the synthesis of three isoforms of alpha-NTX in the venom of a single spitting cobra. DNA amplification by long-distance polymerase chain reaction (LD-PCR) and genome walking have provided information on the gene structure including their promoter and 5' and 3' UTRs. Each NTX isoform is approximately 4 kb in size and contains three exons and two introns. The sequence homology among these isoforms was found to be 99%. Two possible transcription sites were identified by primer extension analysis and they corresponded to the adenine (A) nucleotide at positions +1 and -45. The promoter also contains two TATA boxes and a CCAAT box. Putative binding sites for transcriptional factors AP-2 and GATA are also present. The high percentage of similarity observed among the NTX gene isoforms of N. n. sputatrix as well as with the alpha-NTX and kappa-NTX genes from other land snakes suggests that the NTX gene has probably evolved from a common ancestral gene.
Indexed as
Identifiers
10077532PMC310718W1848587150What OpenQuestion holds
Registered trials
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