ArticleAnnals of surgery1999
Phospholipase A2 mediates nitric oxide production by alveolar macrophages and acute lung injury in pancreatitis.
Article in Annals of surgery, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 62 citations in OpenAlex.
- Lure-and-kill macrophage nanoparticles alleviate the severity of experimental acute pancreatitis.Nature communications · 2021Article
- Autophagy, Inflammation, and Immune Dysfunction in the Pathogenesis of Pancreatitis.Gastroenterology · 2017Review
- Chinese Herbal Medicines Attenuate Acute Pancreatitis: Pharmacological Activities and Mechanisms.Frontiers in pharmacology · 2017Review
- Anti-inflammatory and antioxidant properties of Eriobotrya japonica leaves extracts.African health sciences · 2015Article
- In vitro activity of phospholipase A2 and of peptides from Crotalus durissus terrificus venom against amastigote and promastigote forms of Leishmania (L.) infantum chagasi.The journal of venomous animals and toxins including tropical diseases · 2015Article
- Role of macrophages in the progression of acute pancreatitis.World journal of gastrointestinal pharmacology and therapeutics · 2010Article
- Essential role of monocytes and macrophages in the progression of acute pancreatitis.World journal of gastroenterology · 2010Review
- Discrepancy between the extent of pancreatic necrosis and multiple organ failure score in severe acute pancreatitis.World journal of surgery · 2009Article
- Review
- Article
- Oxidative stress and nitric oxide in rats with alcohol-induced acute pancreatitis.World journal of gastroenterology · 2005Article
- Hypertonic saline enhances host response to bacterial challenge by augmenting receptor-independent neutrophil intracellular superoxide formation.Annals of surgery · 2003Article
- Inhibition of LPS-induced chemokine production in human lung endothelial cells by lipid conjugates anchored to the membrane.British journal of pharmacology · 2002Article
- Involvement of apoptosis of alveolar epithelial cells in acute pancreatitis-associated lung injury.World journal of gastroenterology · 2000Article
- Quantitative measurement of P- and E-selectin adhesion molecules in acute pancreatitis: correlation with distant organ injury.Annals of surgery · 2000Article
- Specific pancreatic enzymes activate macrophages to produce tumor necrosis factor-alpha: role of nuclear factor kappa B and inhibitory kappa B proteins.Journal of gastrointestinal surgery : official journal of the Society for Surgery of the Alimentary TractArticle
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveReportedly, nitric oxide (NO) derived from alveolar macrophages (AMs) and increased serum phospholipase A2 (PLA2) activity are associated with the pathogenesis of lung injury in acute pancreatitis. The authors examined the possibility that PLA2 causes, in part, the induction of NO production by AMs in pancreatitis.
methodsPancreatitis was induced in rats by selective pancreatic duct ligation (SPL). AMs were stimulated with PLA2 or SPL rat serum, with or without administration of the PLA2 inhibitor quinacrine. Then NO production from the AMs was measured by the Griess method, inducible NO synthase mRNA expression of AMs was analyzed by the reverse transcription-polymerase chain reaction, and cytotoxic effects of AMs on human umbilical vein endothelial cells was examined by a 51Cr release assay. In vivo, the effect of quinacrine on lung injury was determined by measuring the arterial blood oxygen pressure (PaO2), lung weight, and lung permeability using Evans blue dye concentration of SPL rat.
resultsIn vitro, the serum with high PLA2 activity induced NO production by rat AMs. PLA2 (50 ng/ml) induced significant amounts of NO production, inducible NO synthase mRNA expression, and cytotoxicity toward the human umbilical vein endothelial cells in normal rat AMs, and these activities were significantly inhibited by quinacrine. In vivo, rats with pancreatitis that were given quinacrine showed decreased concentrations of NO2- and NO3- in the bronchoalveolar lavage fluid, and the PaO2, lung edema, and lung permeability were improved significantly.
conclusionPLA2 induces AMs to release NO, which contributes to lung injury in acute pancreatitis. This lung injury was prevented by the administration of the PLA2 inhibitor quinacrine.
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