ArticleJournal of clinical microbiology1999
Species identification and strain differentiation of dermatophyte fungi by analysis of ribosomal-DNA intergenic spacer regions.
Article in Journal of clinical microbiology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 63 papers, 1 of them a synthesis that pooled it.
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Who cites it
63 citing papers in PubMed, 1 synthesis or guideline pooled it.
- A Comparative Description of Dermatophyte Genomes: A State-of-the-Art Review.Mycopathologia · 2023Pooled it
- First report of tinea corporis caused by Trichophyton quinckeanum in Iran and its antifungal susceptibility profile.Current medical mycology · 2022Article
- Clinical and mycological investigations of post-COVID-19 acute invasive fungal sinusitis.Laryngoscope investigative otolaryngology · 2022Article
- Current Topics in Dermatophyte Classification and Clinical Diagnosis.Pathogens (Basel, Switzerland) · 2022Review
- Dispersal of Aphanoascus keratinophilus by the rook Corvus frugilegus during breeding in East Poland.Scientific reports · 2022Article
- Checkerboard Analysis To Evaluate Synergistic Combinations of Existing Antifungal Drugs and Propylene Glycol Monocaprylate in Isolates from Recalcitrant Tinea Corporis and Cruris Patients Harboring Squalene Epoxidase Gene Mutation.Antimicrobial agents and chemotherapy · 2021Article
- A simple multiplex polymerase chain reaction assay for rapid identification of the common pathogenic dermatophytes: Trichophyton interdigitale, Trichophyton rubrum, and Epidermophyton floccosum.Current medical mycology · 2021Article
- Fungal Genomic Resources for Strain Identification and Diversity Analysis of 1900 Fungal Species.Journal of fungi (Basel, Switzerland) · 2021Article
- Article
- Microbial Diversity and Metabolite Profile of Fermenting Millet in the Production ofFrontiers in microbiology · 2021Article
- A new molecular marker for species-specific identification of Microsporum canis.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2020Article
- Article
- Article
- Detection of Dermatophytes from Dermatophytosis-Suspected Cases in Iran, Evaluation of Polymerase Chain Reaction-Sequencing Method.Advanced biomedical research · 2020Article
- High prevalence of mixed infections in global onychomycosis.PloS one · 2020Article
- FSH1 regulates the phenotype and pathogenicity of the pathogenic dermatophyte Microsporum canis.International journal of molecular medicine · 2019Article
- Potential of fungi isolated from the dumping sites mangrove rhizosphere soil to degrade polythene.Scientific reports · 2019Article
- DNA Barcoding for Identification of Consumer-Relevant Fungi Sold in New York: A Powerful Tool for Citizen Scientists?Foods (Basel, Switzerland) · 2018Article
- DNA barcoding Brooklyn (New York): A first assessment of biodiversity in Marine Park by citizen scientists.PloS one · 2018Article
- Molecular Markers Useful for Intraspecies Subtyping and Strain Differentiation of Dermatophytes.Mycopathologia · 2017Article
3 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Restriction fragment length polymorphisms (RFLPs) identified in the ribosomal-DNA (rDNA) repeat were used for molecular strain differentiation of the dermatophyte fungus Trichophyton rubrum. The polymorphisms were detected by hybridization of EcoRI-digested T. rubrum genomic DNAs with a probe amplified from the small-subunit (18S) rDNA and adjacent internal transcribed spacer (ITS) regions. The rDNA RFLPs mapped to the nontranscribed spacer (NTS) region of the rDNA repeat and appeared similar to those caused by short repetitive sequences in the intergenic spacers of other fungi. Fourteen individual RFLP patterns (DNA types A to N) were recognized among 50 random clinical isolates of T. rubrum. A majority of strains (19 of 50 [38%]) were characterized by one RFLP pattern (DNA type A), and four types (DNA types A to D) accounted for 78% (39 of 50) of all strains. The remaining types (DNA types E to N) were represented by one or two isolates only. A rapid and simple method was also developed for molecular species identification of dermatophyte fungi. The contiguous ITS and 5.8S rDNA regions were amplified from 17 common dermatophyte species by using the universal primers ITS 1 and ITS 4. Digestion of the amplified ITS products with the restriction endonuclease MvaI produced unique and easily identifiable fragment patterns for a majority of species. However, some closely related taxon pairs, such as T. rubrum-T. soudanense and T. quinkeanum-T. schoenlenii could not be distinguished. We conclude that RFLP analysis of the NTS and ITS intergenic regions of the rDNA repeat is a valuable technique both for molecular strain differentiation of T. rubrum and for species identification of common dermatophyte fungi.
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