ArticleJournal of clinical microbiology1999
Serum is more suitable than whole blood for diagnosis of systemic candidiasis by nested PCR.
Article in Journal of clinical microbiology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 116 citations in OpenAlex.
- Prophylaxis of Antifungal Drugs against Systemic Fungemia induced by Oral Candidiasis in Mice.Current issues in molecular biology · 2023Article
- Systemic hematogenous dissemination of mouse oral candidiasis is induced by oral mucositis.Odontology · 2018Article
- A multiplex nested PCR for the detection and identification of Candida species in blood samples of critically ill paediatric patients.BMC infectious diseases · 2014Article
- Multi-probe real-time PCR identification of four common Candida species in blood culture broth.Mycopathologia · 2014Article
- Laboratory diagnosis of invasive aspergillosis: from diagnosis to prediction of outcome.Scientifica · 2013Review
- Comparison of whole blood, serum, and plasma for early detection of candidemia by multiplex-tandem PCR.Journal of clinical microbiology · 2010Article
- Multiplex real-time PCR targeting the RNase P RNA gene for detection and identification of Candida species in blood.Journal of clinical microbiology · 2007Article
- In vitro release by Aspergillus fumigatus of galactofuranose antigens, 1,3-beta-D-glucan, and DNA, surrogate markers used for diagnosis of invasive aspergillosis.Journal of clinical microbiology · 2006Article
- Comparison of different methods of isolation of DNA of commonly encountered Candida species and its quantitation by using a real-time PCR-based assay.Journal of clinical microbiology · 2004Article
- Application of a polymerase chain reaction enzyme immunoassay in peripheral whole blood and serum specimens for diagnosis of acute human brucellosis.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2004Article
- Detection of fungemia by polymerase chain reaction in critically ill neonates and children.Journal of perinatology : official journal of the California Perinatal Association · 2003Article
- Primers ITS1, ITS2 and ITS4 detect the intraspecies variability in the internal transcribed spacers and 5.8S rRNA gene region in clinical isolates of fungi.Folia microbiologica · 2003Article
- Rapid identification of pathogenic fungi directly from cultures by using multiplex PCR.Journal of clinical microbiology · 2002Article
- Current status of nonculture methods for diagnosis of invasive fungal infections.Clinical microbiology reviews · 2002Review
- Identification of Aspergillus fumigatus and related species by nested PCR targeting ribosomal DNA internal transcribed spacer regions.Journal of clinical microbiology · 2001Article
- Serum is the preferred clinical specimen for diagnosis of human brucellosis by PCR.Journal of clinical microbiology · 2001Article
- Comparison between plasma and whole blood specimens for detection of Aspergillus DNA by PCR.Journal of clinical microbiology · 2000Article
- Molecular characterization of Yarrowia lipolytica and Candida zeylanoides isolated from poultry.Applied and environmental microbiology · 2000Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
PCR assays for the diagnosis of systemic candidiasis can be performed either on serum or on whole blood, but results obtained with the two kinds of samples have never been formally compared. Thus we designed a nested PCR assay in which five specific inner pairs of primers were used to amplify specific targets on the rRNA genes of Candida albicans, C. tropicalis, C. parapsilosis, C. krusei, and C. glabrata. In vitro, the lower limit of detection of each nested PCR assay was 1 fg of purified DNA from the corresponding Candida species. In rabbits with candidemia of 120 minutes' duration following intravenous (i.v.) injection of 10(8) CFU of C. albicans, the sensitivities of the PCR in serum and whole blood were not significantly different (93 versus 86%). In other rabbits, injected with only 10(5) CFU of C. albicans, detection of candidemia by culture was possible for only 1 min, whereas DNA could be detected by PCR in whole blood and in serum for 15 and 150 min, respectively. PCR was more often positive in serum than in whole blood in 40 culture-negative samples (27 versus 7%; P < 0.05%). Lastly, experiments with rabbits injected i.v. with 20 or 200 microgram of purified C. albicans DNA showed that PCRs were positive in serum from 30 to at least 120 min after injection, suggesting that the clearance of free DNA is slow. These results suggest that serum is the sample of choice, which should be used preferentially over whole blood for the diagnosis of systemic candidiasis by PCR.
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