ArticleJournal of virology1999
Activation of baculovirus very late promoters by interaction with very late factor 1.
Article in Journal of virology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed.
- Seven Homologous Regions from a NovelInsects · 2026Article
- Specific nucleotide substitutions in the burst sequence enhanceApplied and environmental microbiology · 2025Article
- Continuous Production of Influenza VLPs Using IC-BEVS and Multi-Stage Bioreactors.Biotechnology and bioengineering · 2025Article
- Utility of Alternative Promoters for Foreign Gene Expression Using the Baculovirus Expression Vector System.Viruses · 2022Article
- Article
- A review of alternative promoters for optimal recombinant protein expression in baculovirus-infected insect cells.Protein expression and purification · 2021Review
- Comparison of CRISPR-Cas9 Tools for Transcriptional Repression and Gene Disruption in the BEVS.Viruses · 2021Article
- Whole-genome sequencing and comparative transcriptome analysis of Bombyx mori nucleopolyhedrovirus La strain.Virus genes · 2020Article
- Article
- Review
- Protein composition analysis of polyhedra matrix of Bombyx mori nucleopolyhedrovirus (BmNPV) showed powerful capacity of polyhedra to encapsulate foreign proteins.Scientific reports · 2017Article
- A Conserved Glycine Residue Is Required for Proper Functioning of a Baculovirus VP39 Protein.Journal of virology · 2017Article
- Identification of Essential Genetic Baculoviral Elements for Recombinant Protein Expression by Transactivation in Sf21 Insect Cells.PloS one · 2016Article
- Mutualistic polydnaviruses share essential replication gene functions with pathogenic ancestors.PLoS pathogens · 2013Article
- Analysis of the genome of the sexually transmitted insect virus Helicoverpa zea nudivirus 2.Viruses · 2012Article
- Scalable generation of high-titer recombinant adeno-associated virus type 5 in insect cells.Journal of virology · 2006Article
- Characterization of the role of very late expression factor 1 in baculovirus capsid structure and DNA processing.Journal of virology · 2006Article
- vlf-1 deletion brought AcMNPV to defect in nucleocapsid formation.Virus genes · 2005Article
- Transcriptional activity of baculovirus very late factor 1.Journal of virology · 2005Article
- Baculovirus infection raises the level of TATA-binding protein that colocalizes with viral DNA replication sites.Journal of virology · 2002Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
Very late factor 1 (VLF-1) of Autographa californica multicapsid nuclear polyhedrosis virus (AcMNPV) activates the transcription of two genes, polyhedrin (polh) and p10, during the final, occlusion-specific phase of infection. Using transient expression assays responsive to VLF-1, we identified linker scan mutations in the polh and p10 promoters which abolished or weakened the ability of the promoters to respond to stimulation by VLF-1. These mutations were located between the transcriptional and translational initiation sites, a region previously shown to be essential for the burst of expression during the very late phase. Addition of partially purified, epitope-tagged VLF-1 to DNA encompassing this "burst sequence" resulted in a shift in the gel electrophoretic mobility of the DNA, indicating that VLF-1 forms a complex with DNA. Addition of an antibody specific for the epitope tag of VLF-1 decreased the mobility of the DNA further, confirming the presence of VLF-1 in the complex. DNase I footprint assays revealed that VLF-1 partially purified from either insect cells or bacterial cells interacted with the burst sequences of both the polh and p10 very-late promoters. Linker scan mutations within the burst sequences severely impaired interaction between VLF-1 and the promoters. We propose that VLF-1 transactivates the polh and p10 promoters by interacting with the burst sequences.
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