ArticlePlant physiology1999
GTPase activity and biochemical characterization of a recombinant cotton fiber annexin.
Article in Plant physiology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 95 citations in OpenAlex.
- Function and evolution of plant annexins in response to abiotic stresses.BMC plant biology · 2025Review
- Annexins: central regulators of plant growth and stress signaling.Acta biochimica et biophysica Sinica · 2025Review
- Genome-Wide Identification of theInternational journal of molecular sciences · 2024Article
- Comprehensive analysis of annexin gene family and its expression in response to branching architecture and salt stress in crape myrtle.BMC plant biology · 2024Article
- Genome-Wide Characterization and Abiotic Stresses Expression Analysis of Annexin Family Genes in Poplar.International journal of molecular sciences · 2022Article
- The durum wheat annexin, TdAnn6, improves salt and osmotic stress tolerance in Arabidopsis via modulation of antioxidant machinery.Protoplasma · 2021Article
- Advanced Microscopy Reveals Complex Developmental and Subcellular Localization Patterns of ANNEXIN 1 inFrontiers in plant science · 2020Article
- Analysis of a novel mutant allele of GSL8 reveals its key roles in cytokinesis and symplastic trafficking in Arabidopsis.BMC plant biology · 2018Article
- Moonlighting proteins: putting the spotlight on enzymes.Plant signaling & behavior · 2018Article
- An Overview of Signaling Regulons During Cold Stress Tolerance in Plants.Current genomics · 2017Review
- A Cotton Annexin Affects Fiber Elongation and Secondary Cell Wall Biosynthesis Associated with Ca2+ Influx, ROS Homeostasis, and Actin Filament Reorganization.Plant physiology · 2016Article
- Comprehensive analyses of the annexin gene family in wheat.BMC genomics · 2016Article
- Overexpression of a cotton annexin gene, GhAnn1, enhances drought and salt stress tolerance in transgenic cotton.Plant molecular biology · 2015Article
- Down-regulating annexin gene GhAnn2 inhibits cotton fiber elongation and decreases Ca2+ influx at the cell apex.Plant molecular biology · 2014Article
- Callose homeostasis at plasmodesmata: molecular regulators and developmental relevance.Frontiers in plant science · 2014Article
- Induction of annexin by heavy metals and jasmonic acid in Zea mays.Functional & integrative genomics · 2013Article
- A small-scale proteomic approach reveals a survival strategy, including a reduction in alkaloid biosynthesis, in Hyoscyamus albus roots subjected to iron deficiency.Frontiers in plant science · 2013Article
- Identification and characterization of annexin gene family in rice.Plant cell reports · 2012Article
- Ntann12 annexin expression is induced by auxin in tobacco roots.Journal of experimental botany · 2011Article
- Coping with stresses: roles of calcium- and calcium/calmodulin-regulated gene expression.The Plant cell · 2011Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A cDNA encoding annexin was isolated from a cotton (Gossypium hirsutum) fiber cDNA library. The cDNA was expressed in Escherichia coli, and the resultant recombinant protein was purified. We then investigated some biochemical properties of the recombinant annexin based on the current understanding of plant annexins. An "add-back experiment" was performed to study the effect of the recombinant annexin on beta-glucan synthase activity, but no effect was found. However, it was found that the recombinant annexin could display ATPase/GTPase activities. The recombinant annexin showed much higher GTPase than ATPase activity. Mg2+ was essential for these activities, whereas a high concentration of Ca2+ was inhibitory. A photolabeling assay showed that this annexin could bind GTP more specifically than ATP. The GTP-binding site on the annexin was mapped into the carboxyl-terminal fourth repeat of annexin from the photolabeling experiment using domain-deletion mutants of this annexin. Northern-blot analysis showed that the annexin gene was highly expressed in the elongation stages of cotton fiber differentiation, suggesting a role of this annexin in cell elongation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.