ArticlePlant physiology1999
Osmotic water permeability of isolated protoplasts. Modifications during development
Article in Plant physiology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 79 citations in OpenAlex.
- How sterols affect protoplasts plasma membrane water permeability and their volume under osmotic shock.Journal of plant research · 2025Article
- Phenotyping and modeling of root hydraulic architecture reveal critical determinants of axial water transport.Plant physiology · 2022Article
- Extreme undersaturation in the intercellular airspace of leaves: a failure of Gaastra or Ohm?Annals of botany · 2022Review
- Experimental and conceptual approaches to root water transport.Plant and soil · 2022Review
- Age dependence of the hydraulic resistances of the plasma membrane and the tonoplast (vacuolar membrane) in cells of Chara corallina.Protoplasma · 2021Article
- Aquaporins facilitate hydrogen peroxide entry into guard cells to mediate ABA- and pathogen-triggered stomatal closure.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Aquaporins Contribute to ABA-Triggered Stomatal Closure through OST1-Mediated Phosphorylation.The Plant cell · 2015Article
- Flowers under pressure: ins and outs of turgor regulation in development.Annals of botany · 2014Review
- Measuring the osmotic water permeability coefficient (Pf) of spherical cells: isolated plant protoplasts as an example.Journal of visualized experiments : JoVE · 2014Article
- Arabidopsis SNAREs SYP61 and SYP121 coordinate the trafficking of plasma membrane aquaporin PIP2;7 to modulate the cell membrane water permeability.The Plant cell · 2014Article
- Regulation of leaf hydraulics: from molecular to whole plant levels.Frontiers in plant science · 2013Article
- Selective regulation of maize plasma membrane aquaporin trafficking and activity by the SNARE SYP121.The Plant cell · 2012Article
- A PIP1 aquaporin contributes to hydrostatic pressure-induced water transport in both the root and rosette of Arabidopsis.Plant physiology · 2010Article
- Roles of morphology, anatomy, and aquaporins in determining contrasting hydraulic behavior of roots.Plant physiology · 2009Article
- Rh-PIP2;1, a rose aquaporin gene, is involved in ethylene-regulated petal expansion.Plant physiology · 2008Article
- Article
- Water transport by aquaporins in the extant plant Physcomitrella patens.Plant physiology · 2008Article
- Osmotic water permeability of plasma and vacuolar membranes in protoplasts I: high osmotic water permeability in radish (Raphanus sativus) root cells as measured by a new method.Journal of plant research · 2007Article
- Osmotic water permeability of plasma and vacuolar membranes in protoplasts II: theoretical basis.Journal of plant research · 2007Article
- Measuring the osmotic water permeability of the plant protoplast plasma membrane: implication of the nonosmotic volume.The Journal of membrane biology · 2007Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A transference chamber was developed to measure the osmotic water permeability coefficient (Pos) in protoplasts 40 to 120 μm in diameter. The protoplast was held by a micropipette and submitted to a steep osmotic gradient created in the transference chamber. Pos was derived from the changes in protoplast dimensions, as measured using a light microscope. Permeabilities were in the range 1 to 1000 μm s-1 for the various types of protoplasts tested. The precision for Pos was </=40%, and within this limit, no asymmetry in the water fluxes was observed. Measurements on protoplasts isolated from 2- to 5-d-old roots revealed a dramatic increase in Pos during root development. A shift in Pos from 10 to 500 μm s-1 occurred within less than 48 h. This phenomenon was found in maize (Zea mays), wheat (Triticum aestivum), and rape (Brassica napus) roots. These results show that early developmental processes modify water-transport properties of the plasma membrane, and that the transference chamber is adapted to the study of water-transport mechanisms in native membranes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.