ArticleMolecular biology of the cell1999
Visualization of receptor-mediated endocytosis in yeast.
Article in Molecular biology of the cell, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 74 citations in OpenAlex.
- The cytoplasmic tail of the mechanosensitive channel Pkd2 regulates its internalization and clustering in eisosomes.Journal of cell science · 2023Article
- Electron microscopy for ultrastructural analysis and protein localization inMicrobial cell (Graz, Austria) · 2015Review
- Phosphatidylserine flipping enhances membrane curvature and negative charge required for vesicular transport.The Journal of cell biology · 2013Article
- Down-regulating sphingolipid synthesis increases yeast lifespan.PLoS genetics · 2012Article
- Spatial modeling of vesicle transport and the cytoskeleton: the challenge of hitting the right road.PloS one · 2012Article
- Endocytosis-like protein uptake in the bacterium Gemmata obscuriglobus.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Distinct acto/myosin-I structures associate with endocytic profiles at the plasma membrane.The Journal of cell biology · 2008Article
- The yeast actin cytoskeleton: from cellular function to biochemical mechanism.Microbiology and molecular biology reviews : MMBR · 2006Review
- Spatial dynamics of receptor-mediated endocytic trafficking in budding yeast revealed by using fluorescent alpha-factor derivatives.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- The BAR domain proteins: molding membranes in fission, fusion, and phagy.Microbiology and molecular biology reviews : MMBR · 2006Review
- Actin-based motility during endocytosis in budding yeast.Molecular biology of the cell · 2006Article
- A WASp-binding type II phosphatidylinositol 4-kinase required for actin polymerization-driven endosome motility.The Journal of cell biology · 2005Article
- Review
- The pleckstrin homology domain proteins Slm1 and Slm2 are required for actin cytoskeleton organization in yeast and bind phosphatidylinositol-4,5-bisphosphate and TORC2.Molecular biology of the cell · 2005Article
- Ultrastructural identification of uncoated caveolin-independent early endocytic vehicles.The Journal of cell biology · 2005Article
- Molecular interactions of yeast Neo1p, an essential member of the Drs2 family of aminophospholipid translocases, and its role in membrane trafficking within the endomembrane system.Molecular and cellular biology · 2004Article
- Identification of multivesicular bodies as prevacuolar compartments in Nicotiana tabacum BY-2 cells.The Plant cell · 2004Article
- Tor kinases are in distinct membrane-associated protein complexes in Saccharomyces cerevisiae.Molecular biology of the cell · 2003Article
- In vivo role for actin-regulating kinases in endocytosis and yeast epsin phosphorylation.Molecular biology of the cell · 2001Article
- Ric1p and the Ypt6p GTPase function in a common pathway required for localization of trans-Golgi network membrane proteins.Molecular biology of the cell · 2001Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
We studied the ligand-induced endocytosis of the yeast alpha-factor receptor Ste2p by immuno-electron microscopy. We observed and quantitated time-dependent loss of Ste2p from the plasma membrane of cells exposed to alpha-factor. This ligand-induced internalization of Ste2p was blocked in the well-characterized endocytosis-deficient mutant sac6Delta. We provide evidence that implicates furrow-like invaginations of the plasma membrane as the site of receptor internalization. These invaginations are distinct from the finger-like plasma membrane invaginations within actin cortical patches. Consistent with this, we show that Ste2p is not located within the cortical actin patch before and during receptor-mediated endocytosis. In wild-type cells exposed to alpha-factor we also observed and quantitated a time-dependent accumulation of Ste2p in intracellular, membrane-bound compartments. These compartments have a characteristic electron density but variable shape and size and are often located adjacent to the vacuole. In immuno-electron microscopy experiments these compartments labeled with antibodies directed against the rab5 homologue Ypt51p (Vps21p), the resident vacuolar protease carboxypeptidase Y, and the vacuolar H+-ATPase Vph1p. Using a new double-labeling technique we have colocalized antibodies against Ste2p and carboxypeptidase Y to this compartment, thereby identifying these compartments as prevacuolar late endosomes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.