ArticleMolecular biology of the cell1999
Gene knockouts reveal separate functions for two cytoplasmic dyneins in Tetrahymena thermophila.
Article in Molecular biology of the cell, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 44 citations in OpenAlex.
- FAP206 is a microtubule-docking adapter for ciliary radial spoke 2 and dynein c.Molecular biology of the cell · 2015Article
- Conservation and innovation in Tetrahymena membrane traffic: proteins, lipids, and compartments.Methods in cell biology · 2012Review
- Transcriptome analysis of the model protozoan, Tetrahymena thermophila, using Deep RNA sequencing.PloS one · 2012Article
- Dynein-2 affects the regulation of ciliary length but is not required for ciliogenesis in Tetrahymena thermophila.Molecular biology of the cell · 2009Article
- The Tetrahymena thermophila phagosome proteome.Eukaryotic cell · 2006Article
- Cytoplasmic dynein's mitotic spindle pole localization requires a functional anaphase-promoting complex, gamma-tubulin, and NUDF/LIS1 in Aspergillus nidulans.Molecular biology of the cell · 2005Article
- Dynein is a transient kinetochore component whose binding is regulated by microtubule attachment, not tension.The Journal of cell biology · 2000Article
- RAD51 is required for propagation of the germinal nucleus in Tetrahymena thermophila.Genetics · 2000Article
- Cytoplasmic dynein is required for the nuclear attachment and migration of centrosomes during mitosis in Drosophila.The Journal of cell biology · 1999Article
- Cytoplasmic dynein heavy chain 1b is required for flagellar assembly in Chlamydomonas.Molecular biology of the cell · 1999Article
- Gene knockouts reveal separate functions for two cytoplasmic dyneins in Tetrahymena thermophila.Molecular biology of the cell · 1999Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In many organisms, there are multiple isoforms of cytoplasmic dynein heavy chains, and division of labor among the isoforms would provide a mechanism to regulate dynein function. The targeted disruption of somatic genes in Tetrahymena thermophila presents the opportunity to determine the contributions of individual dynein isoforms in a single cell that expresses multiple dynein heavy chain genes. Substantial portions of two Tetrahymena cytoplasmic dynein heavy chain genes were cloned, and their motor domains were sequenced. Tetrahymena DYH1 encodes the ubiquitous cytoplasmic dynein Dyh1, and DYH2 encodes a second cytoplasmic dynein isoform, Dyh2. The disruption of DYH1, but not DYH2, resulted in cells with two detectable defects: 1) phagocytic activity was inhibited, and 2) the cells failed to distribute their chromosomes correctly during micronuclear mitosis. In contrast, the disruption of DYH2 resulted in a loss of regulation of cell size and cell shape and in the apparent inability of the cells to repair their cortical cytoskeletons. We conclude that the two dyneins perform separate tasks in Tetrahymena.
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