ArticleMolecular biology of the cell1999
Mutations at phosphorylation sites of Xenopus microtubule-associated protein 4 affect its microtubule-binding ability and chromosome movement during mitosis.
Article in Molecular biology of the cell, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
22 citing papers in PubMed, 42 citations in OpenAlex.
- Revisiting the multisite phosphorylation that produces the M-phase supershift of key mitotic regulators.Molecular biology of the cell · 2022Article
- Persistent growth of microtubules at low density.Molecular biology of the cell · 2021Article
- MAP4 as a New Candidate in Cardiovascular Disease.Frontiers in physiology · 2020Review
- Article
- Phosphorylation Dynamics Dominate the Regulated Proteome during Early Xenopus Development.Scientific reports · 2017Article
- Tau-based fluorescent protein fusions to visualize microtubules.Cytoskeleton (Hoboken, N.J.) · 2017Article
- Regulation of microtubule-based transport by MAP4.Molecular biology of the cell · 2014Article
- RNA granule assembly and disassembly modulated by nuclear factor associated with double-stranded RNA 2 and nuclear factor 45.The Journal of biological chemistry · 2014Article
- Cell cycle regulation of microtubule interactomes: multi-layered regulation is critical for the interphase/mitosis transition.Molecular & cellular proteomics : MCP · 2013Article
- MAP4 and CLASP1 operate as a safety mechanism to maintain a stable spindle position in mitosis.Nature cell biology · 2011Article
- Essential role of protein phosphatase 2A in metaphase II arrest and activation of mouse eggs shown by okadaic acid, dominant negative protein phosphatase 2A, and FTY720.The Journal of biological chemistry · 2011Article
- RNA granule protein 140 (RNG140), a paralog of RNG105 localized to distinct RNA granules in neuronal dendrites in the adult vertebrate brain.The Journal of biological chemistry · 2010Article
- Predominant regulators of tubulin monomer-polymer partitioning and their implication for cell polarization.Cellular and molecular life sciences : CMLS · 2009Review
- Article
- A cell number-counting factor regulates levels of a novel protein, SslA, as part of a group size regulation mechanism in Dictyostelium.Eukaryotic cell · 2007Article
- Interphase-specific phosphorylation-mediated regulation of tubulin dimer partitioning in human cells.Molecular biology of the cell · 2007Article
- Mammalian septins regulate microtubule stability through interaction with the microtubule-binding protein MAP4.Molecular biology of the cell · 2005Article
- A novel RNA-binding protein in neuronal RNA granules: regulatory machinery for local translation.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2005Article
- Phosphorylation controls CLIMP-63-mediated anchoring of the endoplasmic reticulum to microtubules.Molecular biology of the cell · 2005Article
- Dynamic recruitment of Cdc2 to specific microtubule structures during mitosis.The Plant cell · 2001Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microtubule-associated proteins (MAPs) bind to and stabilize microtubules (MTs) both in vitro and in vivo and are thought to regulate MT dynamics during the cell cycle. It is known that p220, a major MAP of Xenopus, is phosphorylated by p34(cdc2) kinase as well as MAP kinase in mitotic cells, and that the phosphorylated p220 loses its MT-binding and -stabilizing abilities in vitro. We cloned a full-length cDNA encoding p220, which identified p220 as a Xenopus homologue of MAP4 (XMAP4). To examine the physiological relevance of XMAP4 phosphorylation in vivo, Xenopus A6 cells were transfected with cDNAs encoding wild-type or various XMAP4 mutants fused with a green fluorescent protein. Mutations of serine and threonine residues at p34(cdc2) kinase-specific phosphorylation sites to alanine interfered with mitosis-associated reduction in MT affinity of XMAP4, and their overexpression affected chromosome movement during anaphase A. These findings indicated that phosphorylation of XMAP4 (probably by p34(cdc2) kinase) is responsible for the decrease in its MT-binding and -stabilizing abilities during mitosis, which are important for chromosome movement during anaphase A.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.