ArticleMolecular biology of the cell1999
GCS1, an Arf guanosine triphosphatase-activating protein in Saccharomyces cerevisiae, is required for normal actin cytoskeletal organization in vivo and stimulates actin polymerization in vitro.
Article in Molecular biology of the cell, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 52 citations in OpenAlex.
- Article
- Candida albicans AGE3, the ortholog of the S. cerevisiae ARF-GAP-encoding gene GCS1, is required for hyphal growth and drug resistance.PloS one · 2010Article
- The Arf-GTPase-activating protein Gcs1p is essential for sporulation and regulates the phospholipase D Spo14p.Eukaryotic cell · 2006Article
- Membrane metabolism mediated by Sec14 family members influences Arf GTPase activating protein activity for transport from the trans-Golgi.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Role for Gcs1p in regulation of Arl1p at trans-Golgi compartments.Molecular biology of the cell · 2005Article
- Arf1p provides an unexpected link between COPI vesicles and mRNA in Saccharomyces cerevisiae.Molecular biology of the cell · 2004Article
- "Sleeping beauty": quiescence in Saccharomyces cerevisiae.Microbiology and molecular biology reviews : MMBR · 2004Review
- A role for GEA1 and GEA2 in the organization of the actin cytoskeleton in Saccharomyces cerevisiae.Genetics · 2003Article
- Activity of specific lipid-regulated ADP ribosylation factor-GTPase-activating proteins is required for Sec14p-dependent Golgi secretory function in yeast.Molecular biology of the cell · 2002Article
- Systematic structure-function analysis of the small GTPase Arf1 in yeast.Molecular biology of the cell · 2002Article
- The ADP ribosylation factor-nucleotide exchange factors Gea1p and Gea2p have overlapping, but not redundant functions in retrograde transport from the Golgi to the endoplasmic reticulum.Molecular biology of the cell · 2001Article
- ACAPs are arf6 GTPase-activating proteins that function in the cell periphery.The Journal of cell biology · 2000Article
- Restructuring of focal adhesion plaques by PI 3-kinase. Regulation by PtdIns (3,4,5)-p(3) binding to alpha-actinin.The Journal of cell biology · 2000Article
- The Arf GTPase-activating protein ASAP1 regulates the actin cytoskeleton.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Filling in the GAPs in the ADP-ribosylation factor story.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Sla2p is associated with the yeast cortical actin cytoskeleton via redundant localization signals.Molecular biology of the cell · 1999Article
Corrections and comments
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Authors and funding
8 authors at 5 institutions in 2 countries.
Funding
Abstract
Recent cloning of a rat brain phosphatidylinositol 3,4, 5-trisphosphate binding protein, centaurin alpha, identified a novel gene family based on homology to an amino-terminal zinc-binding domain. In Saccharomyces cerevisiae, the protein with the highest homology to centaurin alpha is Gcs1p, the product of the GCS1 gene. GCS1 was originally identified as a gene conditionally required for the reentry of cells into the cell cycle after stationary phase growth. Gcs1p was previously characterized as a guanosine triphosphatase-activating protein for the small guanosine triphosphatase Arf1, and gcs1 mutants displayed vesicle-trafficking defects. Here, we have shown that similar to centaurin alpha, recombinant Gcs1p bound phosphoinositide-based affinity resins with high affinity and specificity. A novel GCS1 disruption strain (gcs1Delta) exhibited morphological defects, as well as mislocalization of cortical actin patches. gcs1Delta was hypersensitive to the actin monomer-sequestering drug, latrunculin-B. Synthetic lethality was observed between null alleles of GCS1 and SLA2, the gene encoding a protein involved in stabilization of the actin cytoskeleton. In addition, synthetic growth defects were observed between null alleles of GCS1 and SAC6, the gene encoding the yeast fimbrin homologue. Recombinant Gcs1p bound to actin filaments, stimulated actin polymerization, and inhibited actin depolymerization in vitro. These data provide in vivo and in vitro evidence that Gcs1p interacts directly with the actin cytoskeleton in S. cerevisiae.
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Registered trials
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