ArticleMolecular biology of the cell1999
Infrequent translation of a nonsense codon is sufficient to decrease mRNA level.
Article in Molecular biology of the cell, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 29 citations in OpenAlex.
- The position of premature termination codons in the hepatocyte nuclear factor -1 beta gene determines susceptibility to nonsense-mediated decay.Human genetics · 2005Trial
- Premature Termination Codon in 5' Region of Desmoplakin and Plakoglobin Genes May Escape Nonsense-Mediated Decay through the Reinitiation of Translation.International journal of molecular sciences · 2022Article
- Cellular variability of nonsense-mediated mRNA decay.Nature communications · 2021Article
- The Ig heavy chain protein but not its message controls early B cell development.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Pro-B cells sense productive immunoglobulin heavy chain rearrangement irrespective of polypeptide production.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Paired analysis of TCRα and TCRβ chains at the single-cell level in mice.The Journal of clinical investigation · 2011Article
- Nonsense-mediated mRNA decay in human cells: mechanistic insights, functions beyond quality control and the double-life of NMD factors.Cellular and molecular life sciences : CMLS · 2010Review
- Hypoxic inhibition of nonsense-mediated RNA decay regulates gene expression and the integrated stress response.Molecular and cellular biology · 2008Article
- A 3' UTR sequence stabilizes termination codons in the unspliced RNA of Rous sarcoma virus.RNA (New York, N.Y.) · 2006Article
- Kaposi's sarcoma-associated herpesvirus K8beta is derived from a spliced intermediate of K8 pre-mRNA and antagonizes K8alpha (K-bZIP) to induce p21 and p53 and blocks K8alpha-CDK2 interaction.Journal of virology · 2005Article
- Efficient downregulation of immunoglobulin mu mRNA with premature translation-termination codons requires the 5'-half of the VDJ exon.Nucleic acids research · 2004Article
- T-cell receptor sequences that elicit strong down-regulation of premature termination codon-bearing transcripts.The EMBO journal · 2002Article
- Correction of genetic disease by making sense from nonsense.The Journal of clinical investigation · 1999Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In many organisms nonsense mutations decrease the level of mRNA. In the case of mammalian cells, it is still controversial whether translation is required for this nonsense-mediated RNA decrease (NMD). Although previous analyzes have shown that conditions that impede translation termination at nonsense codons also prevent NMD, the residual level of termination was unknown in these experiments. Moreover, the conditions used to impede termination might also have interfered with NMD in other ways. Because of these uncertainties, we have tested the effects of limiting translation of a nonsense codon in a different way, using two mutations in the immunoglobulin mu heavy chain gene. For this purpose we exploited an exceptional nonsense mutation at codon 3, which efficiently terminates translation but nonetheless maintains a high level of mu mRNA. We have shown 1) that translation of Ter462 in the double mutant occurs at only approximately 4% the normal frequency, and 2) that Ter462 in cis with Ter3 can induce NMD. That is, translation of Ter462 at this low (4%) frequency is sufficient to induce NMD.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.