ArticleThe Journal of physiology1999
In vitro simultaneous measurements of relaxation and nitric oxide concentration in rat superior mesenteric artery.
Article in The Journal of physiology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 120 citations in OpenAlex.
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- Soluble guanylate cyclase mediates the relaxation of healthy and inflamed bladder smooth muscle by aqueous nitric oxide.Frontiers in physiology · 2023Article
- Tracking endothelium-dependent NO release in pressurized arteries.Frontiers in physiology · 2023Article
- The Transient Receptor Potential Vanilloid 4 Channel and Cardiovascular Disease Risk Factors.Frontiers in physiology · 2021Review
- Enzyme Prodrug Therapy Achieves Site-Specific, Personalized Physiological Responses to the Locally Produced Nitric Oxide.ACS applied materials & interfaces · 2018Article
- Article
- Novel selective PDE type 1 inhibitors cause vasodilatation and lower blood pressure in rats.British journal of pharmacology · 2017Article
- Alterations in perivascular innervation function in mesenteric arteries from offspring of diabetic rats.British journal of pharmacology · 2015Article
- Characterisation and comparison of temporal release profiles of nitric oxide generating donors.Journal of neuroscience methods · 2015Article
- Autoregulation and mechanotransduction control the arteriolar response to small changes in hematocrit.American journal of physiology. Heart and circulatory physiology · 2012Article
- Combating oxidative stress in vascular disease: NADPH oxidases as therapeutic targets.Nature reviews. Drug discovery · 2011Review
- Openers of small conductance calcium-activated potassium channels selectively enhance NO-mediated bradykinin vasodilatation in porcine retinal arterioles.British journal of pharmacology · 2010Article
- Hydrosulfide (HS-) coordination in iron porphyrinates.Inorganic chemistry · 2010Article
- A mathematical model of vasoreactivity in rat mesenteric arterioles: I. Myoendothelial communication.Microcirculation (New York, N.Y. : 1994) · 2009Article
- Real-time dynamics of nitric oxide shifts within the esophageal wall.Surgical endoscopy · 2009Article
- What is the real physiological NO concentration in vivo?Nitric oxide : biology and chemistry · 2009Review
- Inhibition of p38 MAPK reverses hypoxia-induced pulmonary artery endothelial dysfunction.American journal of physiology. Heart and circulatory physiology · 2009Article
- Independent regulation of periarteriolar and perivenular nitric oxide mechanisms in the in vivo hamster cheek pouch microvasculature.Microcirculation (New York, N.Y. : 1994) · 2009Article
- Impaired coronary microvascular dilation correlates with enhanced vascular smooth muscle MLC phosphorylation in diabetes.Microcirculation (New York, N.Y. : 1994) · 2009Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
1. The relationship between nitric oxide (NO) concentration measured with an NO-specific microelectrode and endothelium-dependent relaxation was investigated in isolated rat superior mesenteric artery contracted with 1 microM noradrenaline. 2. Acetylcholine (10 microM) induced endothelium-dependent simultaneous increases in luminal NO concentration of 21 +/- 6 nM, and relaxations with pD2 values and maximum of 6.95 +/- 0.32 and 97.5 +/- 0.7 % (n = 7), respectively. An inhibitor of NO synthase, N G-nitro-L-arginine (L-NOARG, 100 microM) inhibited the relaxations and increases in NO concentration induced by acetylcholine. 3. Oxyhaemoglobin (10 microM) reversed the relaxations and increases in NO concentrations induced by acetylcholine, S-nitroso-N-acetylpenicillamine (SNAP) and S-morpholino-sydnonimine (SIN-1), but not the relaxations induced with forskolin. Oxyhaemoglobin also decreased the NO concentration below baseline level. 4. In the presence of L-NOARG (100 microM), a small relaxation to acetylcholine (10 microM) of noradrenaline-contracted segments was still seen; oxyhaemogobin inhibited this relaxation and decreased the NO concentration by 14 +/- 4 nM (n = 4). 5. The NO concentration-relaxation relationship for acetylcholine resembled that for SNAP and SIN-1 more than for authentic NO. Thus while 7-17 nM NO induced half-maximal relaxations in response to SNAP or SIN-1, 378 +/- 129 nM NO (n = 4) was needed for half-maximal relaxation to authentic NO. 6. The present study provides direct evidence that the relaxation of the rat superior mesenteric artery with the endothelium-dependent vasodilator acetylcholine is correlated to the endogeneous release of NO. The study also suggests that NO mediates the L-NOARG-resistant relaxations in this artery, and that there is a basal NO release.
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