ArticleThe Journal of physiology1999
Relationship between L-type Ca2+ current and unitary sarcoplasmic reticulum Ca2+ release events in rat ventricular myocytes.
Article in The Journal of physiology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- Review
- CaJournal of molecular and cellular cardiology · 2023Article
- Mechanisms and physiological implications of cooperative gating of clustered ion channels.Physiological reviews · 2022Review
- Improper Remodeling of Organelles Deputed to CaInternational journal of molecular sciences · 2021Review
- Interplay among distinct CaThe Journal of physiology · 2017Article
- Ca2+ channel nanodomains boost local Ca2+ amplitude.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Maternal high-altitude hypoxia and suppression of ryanodine receptor-mediated Ca2+ sparks in fetal sheep pulmonary arterial myocytes.American journal of physiology. Lung cellular and molecular physiology · 2012Article
- Excitation-contraction coupling gain in ventricular myocytes: insights from a parsimonious model.The Journal of physiology · 2009Article
- Indirect coupling between Cav1.2 channels and ryanodine receptors to generate Ca2+ sparks in murine arterial smooth muscle cells.The Journal of physiology · 2007Article
- Ca2+-sparks constitute elementary building blocks for global Ca2+-signals in myocytes of retinal arterioles.Cell calcium · 2007Article
- Ca2+ -induced Ca2+ release through localized Ca2+ uncaging in smooth muscle.The Journal of general physiology · 2006Article
- Variability in couplon size in rabbit ventricular myocytes.Biophysical journal · 2005Article
- Local recovery of Ca2+ release in rat ventricular myocytes.The Journal of physiology · 2005Article
- Coupled dynamics of voltage and calcium in paced cardiac cells.Physical review. E, Statistical, nonlinear, and soft matter physics · 2005Article
- Model of intracellular calcium cycling in ventricular myocytes.Biophysical journal · 2003Article
- Effects of caffeine on potassium currents in isolated rat ventricular myocytes.Pflugers Archiv : European journal of physiology · 2003Article
- Regulation of junctional and non-junctional sarcoplasmic reticulum calcium release in excitation-contraction coupling in cat atrial myocytes.The Journal of physiology · 2003Article
- Regulation of cardiac excitation-contraction coupling by action potential repolarization: role of the transient outward potassium current (I(to)).The Journal of physiology · 2003Review
- An integrative model of the cardiac ventricular myocyte incorporating local control of Ca2+ release.Biophysical journal · 2002Article
- Spontaneous transient outward currents arise from microdomains where BK channels are exposed to a mean Ca(2+) concentration on the order of 10 microM during a Ca(2+) spark.The Journal of general physiology · 2002Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
1. The time courses of Ca2+ current and Ca2+ spark occurrence were determined in single rat ventricular myocytes voltage clamped with patch pipettes containing 0.1 microM fluo-3. Acquisition of line-scan images on a laser scanning confocal microscope was synchronized with measurement of Cd2+-sensitive Ca2+ currents. In most cells, individual Ca2+ sparks were observed by reducing Ca2+ current density with nifedipine (0.1-8 microM). 2. Ca2+ sparks elicited by depolarizing voltage-clamp pulses had a peak [Ca2+] amplitude of 289 +/- 3 nM with a decay half-time of 20.8 +/- 0.2 ms and a full width at half-maximum of 1.40 +/- 0.03 microm (mean +/- s. e.m., n = 345), independent of the membrane potential. 3. The time between the beginning of a depolarization and the initiation of each Ca2+ spark was calculated and data were pooled to construct waiting time histograms. Exponential functions were fitted to these histograms and to the decaying phase of the Ca2+ current. This analysis showed that the time constants describing Ca2+ current and Ca2+ spark occurrence at membrane potentials between -30 mV and +30 mV were not significantly different. At +50 mV, in the absence of nifedipine, the time constant describing Ca2+ spark occurrence was significantly larger than the time constant of the Ca2+ current. 4. A simple model is developed using Poisson statistics to relate macroscopic Ca2+ current to the opening of single L-type Ca2+ channels at the dyad junction and to the time course of Ca2+ spark occurrence. The model suggests that the time courses of macroscopic Ca2+ current and Ca2+ spark occurrence should be closely related when opening of a single L-type Ca2+ channel initiates a Ca2+ spark. By comparison with the data, the model suggests that Ca2+ sparks are initiated by the opening of a single L-type Ca2+ channel at all membrane potentials encountered during an action potential.
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