ArticleThe Journal of physiology1999
Differential plasma membrane targeting of voltage-dependent calcium channel subunits expressed in a polarized epithelial cell line.
Article in The Journal of physiology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 52 citations in OpenAlex.
- Regulation of Presynaptic Calcium Channels.Advances in neurobiology · 2023Article
- Review
- Calmodulin Antagonist W-7 Enhances Intermediate Conductance CaThe Journal of membrane biology · 2021Article
- A potent voltage-gated calcium channel inhibitor engineered from a nanobody targeted to auxiliary CaeLife · 2019Article
- Trafficking of neuronal calcium channels.Neuronal signaling · 2017Review
- Vitamin D3 suppresses morphological evolution of the cribriform cancerous phenotype.Oncotarget · 2016Article
- The role of auxiliary subunits for the functional diversity of voltage-gated calcium channels.Journal of cellular physiology · 2015Review
- Age-dependent contribution of P/Q- and R-type Ca2+ channels to neuromuscular transmission in lethargic mice.The Journal of pharmacology and experimental therapeutics · 2015Article
- The Ca2+ channel β2 subunit is selectively targeted to the axon terminals of supraoptic neurons.Channels (Austin, Tex.) · 2014Article
- Different subcellular populations of L-type Ca2+ channels exhibit unique regulation and functional roles in cardiomyocytes.Journal of molecular and cellular cardiology · 2012Review
- The ß subunit of voltage-gated Ca2+ channels.Physiological reviews · 2010Review
- Differential modulation of N-type calcium channels by micro-opioid receptors in oxytocinergic versus vasopressinergic neurohypophysial terminals.Journal of cellular physiology · 2010Article
- Genomic organization, expression, and phylogenetic analysis of Ca2+ channel beta4 genes in 13 vertebrate species.Physiological genomics · 2008Article
- Localization and targeting of voltage-dependent ion channels in mammalian central neurons.Physiological reviews · 2008Review
- Novel splice variants of rat CaV2.1 that lack much of the synaptic protein interaction site are expressed in neuroendocrine cells.The Journal of biological chemistry · 2008Article
- Dominant-negative suppression of Cav2.1 currents by alpha(1)2.1 truncations requires the conserved interaction domain for beta subunits.Molecular and cellular neurosciences · 2007Article
- Cellular localization of voltage-gated calcium channels and synaptic vesicle-associated proteins in the guinea pig cochlea.Journal of molecular neuroscience : MN · 2005Article
- Ca2+ channels as targets of neurological disease: Lambert-Eaton Syndrome and other Ca2+ channelopathies.Journal of bioenergetics and biomembranes · 2003Review
- Beta subunits of voltage-gated calcium channels.Journal of bioenergetics and biomembranes · 2003Review
- Targeting mechanisms of high voltage-activated Ca2+ channels.Journal of bioenergetics and biomembranes · 2003Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
1. Voltage-dependent calcium channels (VDCCs) show a highly non-uniform distribution in many cell types, including neurons and other polarized secretory cells. We have examined whether this can be mimicked in a polarized epithelial cell line (Madin-Darby canine kidney), which has been used extensively to study the targeting of proteins. 2. We expressed the VDCC alpha1A, alpha1B or alpha1C subunits either alone or in combination with accessory subunits alpha2-delta and the different beta subunits, and examined their localization immunocytochemically. An alpha1 subunit was only targeted to the plasma membrane if co-expressed with the accessory subunits. 3. The combination alpha1C/alpha2-delta and all beta subunits was always localized predominantly to the basolateral membrane. It has been suggested that this is equivalent to somatodendritic targeting in neurons. 4. In contrast, the alpha1B subunit was expressed at the apical membrane with all the accessory subunit combinations, by 24 h after microinjection. This membrane destination shows some parallels with axonal targeting in neurons. 5. The alpha1A subunit was consistently observed at the apical membrane in the combinations alpha1A/alpha2-delta/beta1b or beta4. In contrast, when co-expressed with alpha2-delta/beta2a, alpha1A was clearly targeted to the basolateral membrane. 6. In conclusion, the VDCC alpha1 subunit appears to be the primary determinant for targeting the VDCC complex, but the beta subunit can modify this destination, particularly for alpha1A.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.