Evidence map›Paper›PMID 10066896›Full record

ArticleThe Journal of physiology1999

Regulation of an amiloride-sensitive Na+-permeable channel by a beta2-adrenergic agonist, cytosolic Ca2+ and Cl- in fetal rat alveolar epithelium.

Y Marunaka, N Niisato, H O'Brodovich, D C Eaton

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of physiology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.

0numbers the graph read from it
0cells of the map it votes in
20citing papers in PubMed
3.8field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

20 citing papers in PubMed, 74 citations in OpenAlex.

  1. Physiological roles of chloride ions in bodily and cellular functions.The journal of physiological sciences : JPS · 2023
    Review
  2. Article
  3. Article
  4. Article
  5. Article
  6. ENaC is regulated by natriuretic peptide receptor-dependent cGMP signaling.American journal of physiology. Renal physiology · 2013
    Article
  7. Nasal potential difference to detect Na+ channel dysfunction in acute lung injury.American journal of physiology. Lung cellular and molecular physiology · 2011
    Review
  8. Regulation of epithelial sodium transport via epithelial Na+ channel.Journal of biomedicine & biotechnology · 2011
    Review
  9. Article
  10. Article
  11. Article
  12. Article
  13. Alveolar epithelial ion and fluid transport: recent progress.American journal of respiratory cell and molecular biology · 2006
    Review
  14. Article
  15. Article
  16. Article
  17. Article
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 2 countries.

Y MarunakaThe Hospital for Sick Children Research Institute, Department of Pediatrics and Institute of Medical Science, The University of Toronto Faculty of Medicine, Toronto, Ontario, Canada M5G 1X8. marunaka@sickkids.on.ca
N Niisato
H O'Brodovich
D C Eaton
University of Toronto · CAEmory University · US

Funding

REGULATION OF SODIUM IN TIGHT EPITHELIAR37DK037963 · NIDDK · EMORY UNIVERSITY · PI EATON, DOUGLAS C. · 2002 to 2011
$3.8M
REGULATION OF SODIUM TRANSPORT IN TIGHT EPITHELIAR01DK037963 · NIDDK · EMORY UNIVERSITY · PI EATON, DOUGLAS C · 1987 to 2001
$496k
NIDDK NIH HHS DK37963NIDDK NIH HHS R01 DK037963NIDDK NIH HHS R37 DK037963
6 · The paper itself

Abstract

1. In cell-attached patches formed on the apical membrane of fetal alveolar epithelium, terbutaline (a specific beta2-adrenergic agonist) increased the open probability (Po) of an amiloride-sensitive Na+-permeable non-selective cation (NSC) channel (control, 0.03 +/- 0.04; terbutaline, 0.62 +/- 0.18; n = 8, P < 0. 00001) by increasing the mean open time 100-fold without any significant change in the mean closed time and without any change in the single channel conductance (control, 27.8 +/- 2.3 pS; terbutaline, 28.2 +/- 2.1 pS; n = 8). 2. The Po of the unstimulated channel increased when the apical membrane was depolarized due to a decrease in the closing rate and an increase in the opening rate, while the Po of the terbutaline-stimulated channel did not depend on the membrane potential. 3. Increased cytosolic [Ca2+] also increased the Po of the channel in a manner consistent with one Ca2+-binding site on the cytosolic surface of the channel. Terbutaline increased the sensitivity of the channel to cytosolic Ca2+ by shifting the concentration of cytosolic Ca2+ ([Ca2+]c) required for half-maximal activation to a lower [Ca2+]c value, leading to an increase in Po. 4. An increase in the cytosolic Cl- concentration ([Cl-]c) decreased the Po of the channel consistent with two Cl--binding sites by increasing the closing rate without any significant change in the opening rate. Terbutaline increased Po by reducing the effect of cytosolic Cl- to promote channel closing. 5. Taken together, these observations indicate that terbutaline activates a Ca2+-activated, Cl--inhibitable, amiloride-sensitive, Na+-permeable NSC channel in fetal rat alveolar epithelium in two ways: first, through an increase in Ca2+ sensitivity, and second, through a reduction in the effect of cytosolic Cl- to promote channel closing.

Indexed as

Adrenergic beta-AgonistsAmilorideAnimalsCalciumCells, CulturedChloridesCytosolEpithelial CellsFetusIon Channel GatingMembrane PotentialsModels, BiologicalPatch-Clamp TechniquesPulmonary AlveoliRatsRats, WistarAdrenergic beta-AgonistsAmilorideCalciumChloridesSodium ChannelsTerbutaline

Identifiers

PMID10066896
PMCPMC2269183
OpenAlexW1973901120

What OpenQuestion holds

Texttitle and abstract
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.