ArticleThe Journal of physiology1999
Selective activation of heterologously expressed G protein-gated K+ channels by M2 muscarinic receptors in rat sympathetic neurones.
Article in The Journal of physiology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed, 55 citations in OpenAlex.
- Presynaptic Adrenoceptors.Handbook of experimental pharmacology · 2024Review
- Muscarinic Receptor Stimulation Does Not Inhibit Voltage-dependent CaActa histochemica et cytochemica · 2023Article
- Muscarinic receptors in adrenal chromaffin cells: physiological role and regulation of ion channels.Pflugers Archiv : European journal of physiology · 2018Review
- Knockouts reveal overlapping functions of M(2) and M(4) muscarinic receptors and evidence for a local glutamatergic circuit within the laterodorsal tegmental nucleus.Journal of neurophysiology · 2012Article
- Countervailing modulation of Ih by neuropeptide Y and corticotrophin-releasing factor in basolateral amygdala as a possible mechanism for their effects on stress-related behaviors.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2010Article
- The scaffold protein NHERF2 determines the coupling of P2Y1 nucleotide and mGluR5 glutamate receptor to different ion channels in neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2010Article
- Muscarinic acetylcholine receptors (mAChRs) in the nervous system: some functions and mechanisms.Journal of molecular neuroscience : MN · 2010Review
- Differential Inhibition of Ca2+ channels by alpha2-adrenoceptors in three functional subclasses of rat sympathetic neurons.Journal of neurophysiology · 2008Article
- Modulation of pain transmission by G-protein-coupled receptors.Pharmacology & therapeutics · 2008Review
- Peptide YY(3-36) inhibits both anorexigenic proopiomelanocortin and orexigenic neuropeptide Y neurons: implications for hypothalamic regulation of energy homeostasis.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2005Article
- Relationship between membrane phosphatidylinositol-4,5-bisphosphate and receptor-mediated inhibition of native neuronal M channels.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2005Article
- Regulation of rat mesencephalic GABAergic neurones through muscarinic receptors.The Journal of physiology · 2004Article
- The plasma membrane calcium-ATPase as a major mechanism for intracellular calcium regulation in neurones from the rat superior cervical ganglion.The Journal of physiology · 2003Article
- Pharmacological discrimination between muscarinic receptor signal transduction cascades with bethanechol chloride.British journal of pharmacology · 2003Article
- A pervasive mechanism for analgesia: activation of GIRK2 channels.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Gating properties of GIRK channels activated by Galpha(o)- and Galpha(i)-coupled muscarinic m2 receptors in Xenopus oocytes: the role of receptor precoupling in RGS modulation.The Journal of physiology · 2002Article
- Functional expression and FRET analysis of green fluorescent proteins fused to G-protein subunits in rat sympathetic neurons.The Journal of physiology · 2001Article
- Differential regulation of G protein-gated inwardly rectifying K(+) channel kinetics by distinct domains of RGS8.The Journal of physiology · 2001Article
- A herpes simplex viral vector expressing green fluorescent protein can be used to visualize morphological changes in high-density neuronal culture.Electronic journal of biotechnology : EJB · 2001Article
- RGS2 blocks slow muscarinic inhibition of N-type Ca(2+) channels reconstituted in a human cell line.The Journal of physiology · 2001Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
1. G protein-regulated inward rectifier K+ (GIRK) channels were over-expressed in dissociated rat superior cervical sympathetic (SCG) neurones by co-transfecting green fluorescent protein (GFP)-, GIRK1- and GIRK2-expressing plasmids using the biolistic technique. Membrane currents were subsequently recorded with whole-cell patch electrodes. 2. Co-transfected cells had larger Ba2+-sensitive inwardly rectifying currents and 13 mV more negative resting potentials (in 3 mM [K+]o) than non-transfected cells, or cells transfected with GIRK1 or GIRK2 alone. 3. Carbachol (CCh, 1-30 microM) increased the inwardly rectifying current in 70 % of GIRK1+ GIRK2-transfected cells by 261 +/- 53 % (n = 6, CCh 30 microM) at -120 mV, but had no effect in non-transfected cells or in cells transfected with GIRK1 or GIRK2 alone. Pertussis toxin prevented the effect of carbachol but had no effect on basal currents. 4. The effect of CCh was antagonized by 6 nM tripitramine but not by 100 nM pirenzepine, consistent with activation of endogenous M2 muscarinic acetylcholine receptors. 5. In contrast, inhibition of the voltage-activated Ca2+ current by CCh was antagonized by 100 nM pirenzepine but not by 6 nM tripitramine, indicating that it was mediated by M4 muscarinic acetylcholine receptors. 6. We conclude that endogenous M2 and M4 muscarinic receptors selectively couple to GIRK currents and Ca2+ currents respectively, with negligible cross-talk.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.