ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience1999
Metabolic stabilization of muscle nicotinic acetylcholine receptor by rapsyn.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 44 citations in OpenAlex.
- An Inside Job: Molecular Determinants for Postsynaptic Localization of Nicotinic Acetylcholine Receptors.Molecules (Basel, Switzerland) · 2021Review
- HDAC6 regulates microtubule stability and clustering of AChRs at neuromuscular junctions.The Journal of cell biology · 2020Article
- Determinants in the β and δ subunit cytoplasmic loop regulate Golgi trafficking and surface expression of the muscle acetylcholine receptor.The Journal of biological chemistry · 2014Article
- Article
- Cholesterol modulates the rate and mechanism of acetylcholine receptor internalization.The Journal of biological chemistry · 2011Article
- Rapsyn interacts with the muscle acetylcholine receptor via alpha-helical domains in the alpha, beta, and epsilon subunit intracellular loops.Neuroscience · 2009Article
- Receptor-associated proteins and synaptic plasticity.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2009Review
- HSP90 beta regulates rapsyn turnover and subsequent AChR cluster formation and maintenance.Neuron · 2008Article
- Localized acetylcholine receptor clustering dynamics in response to microfluidic focal stimulation with agrin.Biophysical journal · 2008Article
- Rapsyn carboxyl terminal domains mediate muscle specific kinase-induced phosphorylation of the muscle acetylcholine receptor.Neuroscience · 2008Article
- Regulation of nicotinic receptor trafficking by the transmembrane Golgi protein UNC-50.The EMBO journal · 2007Article
- Overexpression of rapsyn in rat muscle increases acetylcholine receptor levels in chronic experimental autoimmune myasthenia gravis.The American journal of pathology · 2007Article
- Increased ratio of rapsyn to ACh receptor stabilizes postsynaptic receptors at the mouse neuromuscular synapse.The Journal of physiology · 2005Article
- Rapsyn escorts the nicotinic acetylcholine receptor along the exocytic pathway via association with lipid rafts.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2002Article
- Acetylcholine receptors are required for agrin-induced clustering of postsynaptic proteins.The EMBO journal · 2001Article
- Dendritic and axonal targeting of type 5 metabotropic glutamate receptor is regulated by homer1 proteins and neuronal excitation.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2000Article
- Long-term nicotine adaptation in Caenorhabditis elegans involves PKC-dependent changes in nicotinic receptor abundance.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2000Article
- Mutation causing congenital myasthenia reveals acetylcholine receptor beta/delta subunit interaction essential for assembly.The Journal of clinical investigation · 1999Article
- Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Although the metabolic half-life of muscle endplate acetylcholine receptor (AChR) changes during development and after denervation in the adult, little is known about the molecular mechanisms that influence receptor stability. We have investigated the effect on AChR turnover of its interaction with rapsyn, a 43 kDa peripheral membrane protein that is closely associated with the AChR in muscle cells and is required for its clustering at endplates. Both in transfected COS cells and in cultured myotubes from rapsyn-negative and rapsyn-positive mice, we have found that the presence of rapsyn slows the turnover of AChRs by as much as twofold. The effect was similar for both embryonic (alpha2betadeltagamma) and adult (alpha2betadeltaepsilon) AChRs and for AChRs whose beta subunit lacked a putative tyrosine phosphorylation site. Neither colchicine nor cytochalasin D altered AChR turnover or prevented the rapsyn effect. Mutant rapsyn proteins whose N-terminal myristoylation signal was eliminated, or whose C terminus or zinc-finger domains were deleted, failed to change the rate of receptor turnover. Each of these mutations affects the association of the AChR with rapsyn, suggesting that AChR stability is altered by interaction between the two proteins. Our results suggest that, in addition to its role in AChR clustering, rapsyn also functions to metabolically stabilize the AChR.
Indexed as
Identifiers
10066253PMC6782578W1899336325What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.