ArticleProceedings of the National Academy of Sciences of the United States of America1999
Continuous axenic cultivation of Pneumocystis carinii.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
32 citing papers in PubMed.
- Article
- Selective expression ofmBio · 2025Article
- Metabolic modelling as a powerful tool to identify critical components of Pneumocystis growth medium.PLoS computational biology · 2024Article
- Fungal pathogens and symbionts: Living off the fat of the land.PLoS pathogens · 2024Review
- Axenic Long-Term Cultivation ofJournal of fungi (Basel, Switzerland) · 2023Article
- The Persistent Challenge of Pneumocystis Growth Outside the Mammalian Lung: Past and Future Approaches.Frontiers in microbiology · 2021Review
- Metagenomic next-generation sequencing for mixed pulmonary infection diagnosis.BMC pulmonary medicine · 2019Article
- Observational
- SYTO-13, a Viability Marker as a New Tool to Monitor In Vitro Pharmacodynamic Parameters of Anti-Pneumocystis Drugs.PloS one · 2015Article
- Pneumocystis.Cold Spring Harbor perspectives in medicine · 2014Review
- Update on the diagnosis and treatment of Pneumocystis pneumonia.Therapeutic advances in respiratory disease · 2011Review
- Biofilm formation by Pneumocystis spp.Eukaryotic cell · 2009Article
- Pneumocystis encodes a functional S-adenosylmethionine synthetase gene.Eukaryotic cell · 2008Article
- Pneumocystis carinii exhibits a conserved meiotic control pathway.Infection and immunity · 2008Article
- Gene arrays at Pneumocystis carinii telomeres.Genetics · 2005Article
- Ubiquinone synthesis in mitochondrial and microsomal subcellular fractions of Pneumocystis spp.: differential sensitivities to atovaquone.Eukaryotic cell · 2005Article
- Sterol metabolism in the opportunistic pathogen Pneumocystis: advances and new insights.Lipids · 2004Review
- Lung epithelial cells and extracellular matrix components induce expression of Pneumocystis carinii STE20, a gene complementing the mating and pseudohyphal growth defects of STE20 mutant yeast.Infection and immunity · 2003Article
- Surfactant protein D-mediated aggregation of Pneumocystis carinii impairs phagocytosis by alveolar macrophages.Infection and immunity · 2003Article
- Diversity at the locus associated with transcription of a variable surface antigen of Pneumocystis carinii as an index of population structure and dynamics in infected rats.Infection and immunity · 2003Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Continuous axenic culture of Pneumocystis carinii has been achieved. A culture vessel is used that allows for frequent medium exchange without disturbance of organisms that grow attached to a collagen-coated porous membrane. The growth medium is based on Minimal Essential Medium with Earle's salt supplemented with S-adenosyl-L-methionine, putrescine, ferric pyrophosphate, N-acetyl glucosamine, putrescine, p-aminobenzoic acid, L-cysteine and L-glutamine, and horse serum. Incubation is in room air at 31 degrees C. The pH of the medium begins at 8.8 and rises to approximately 9 as the cells grow. Doubling times calculated from growth curves obtained from cultures inoculated at moderate densities ranged from 35 to 65 hours. With a low-density inoculum, the doubling time is reduced to 19 hours. The morphology of cultured organisms in stained smears and in transmission electron micrographs is that of P. carinii, and P. carinii-specific mAbs label the cultured material. Cultured organisms are infective for immunosuppressed rats and can be stored frozen and used to reinitiate culture.
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Registered trials
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