ArticleProceedings of the National Academy of Sciences of the United States of America1999
Imaging adenoviral-directed reporter gene expression in living animals with positron emission tomography.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 103 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
103 citing papers in PubMed, 457 citations in OpenAlex.
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- BRET Sensors for Imaging Membrane Integrity of Microfluidically Generated Extracellular Vesicles.Methods in molecular biology (Clifton, N.J.) · 2022Article
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- Applications of nuclear-based imaging in gene and cell therapy: probe considerations.Molecular therapy oncolytics · 2021Article
- The reduction ofPhysics in medicine and biology · 2020Article
- Interrogating Cellular Communication in Cancer with Genetically Encoded Imaging Reporters.Radiology. Imaging cancer · 2020Review
- Molecular Imaging with Reporter Genes: Has Its Promise Been Delivered?Journal of nuclear medicine : official publication, Society of Nuclear Medicine · 2019Review
- Positron emission tomography reporter gene strategy for use in the central nervous system.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Engineering molecular imaging strategies for regenerative medicine.Bioengineering & translational medicine · 2018Review
- Molecular Imaging in Synthetic Biology, and Synthetic Biology in Molecular Imaging.Molecular imaging and biology · 2017Review
- Innovative reporter gene imaging techniques making inroads to biology.Cardiovascular research · 2016Article
- Stem Cell Monitoring with a Direct or Indirect Labeling Method.Nuclear medicine and molecular imaging · 2016Review
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43 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
14 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We are developing quantitative assays to repeatedly and noninvasively image expression of reporter genes in living animals, using positron emission tomography (PET). We synthesized positron-emitting 8-[18F]fluoroganciclovir (FGCV) and demonstrated that this compound is a substrate for the herpes simplex virus 1 thymidine kinase enzyme (HSV1-TK). Using positron-emitting FGCV as a PET reporter probe, we imaged adenovirus-directed hepatic expression of the HSV1-tk reporter gene in living mice. There is a significant positive correlation between the percent injected dose of FGCV retained per gram of liver and the levels of hepatic HSV1-tk reporter gene expression (r2 > 0.80). Over a similar range of HSV1-tk expression in vivo, the percent injected dose retained per gram of liver was 0-23% for ganciclovir and 0-3% for FGCV. Repeated, noninvasive, and quantitative imaging of PET reporter gene expression should be a valuable tool for studies of human gene therapy, of organ/cell transplantation, and of both environmental and behavioral modulation of gene expression in transgenic mice.
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Registered trials
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