ArticleProceedings of the National Academy of Sciences of the United States of America1999
Predominant VH genes expressed in innate antibodies are associated with distinctive antigen-binding sites.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 49 citations in OpenAlex.
- Phosphatidylcholine-specific B cells are enriched among atypical CD11cFrontiers in immunology · 2025Article
- B1-cell-produced anti-phosphatidylserine antibodies contribute to lupus nephritis development via TLR-mediated Syk activation.Cellular & molecular immunology · 2023Article
- T-betJournal of immunology (Baltimore, Md. : 1950) · 2022Article
- Age-related changes in antigen-specific natural antibodies are influenced by sex.Frontiers in immunology · 2022Article
- A Germline-Encoded Structural Arginine Trap Underlies the Anti-DNA Reactivity of a Murine V Gene Segment.International journal of molecular sciences · 2021Article
- Antigen Receptor Specificity and Cell Location Influence the Diversification and Selection of the B-1a Cell Pool with Age.Journal of immunology (Baltimore, Md. : 1950) · 2020Article
- Massively Parallel Sequencing of Peritoneal and Splenic B Cell Repertoires Highlights Unique Properties of B-1 Cell Antibodies.Journal of immunology (Baltimore, Md. : 1950) · 2018Article
- A Hard(y) Look at B-1 Cell Development and Function.Journal of immunology (Baltimore, Md. : 1950) · 2017Review
- BTK inhibition results in impaired CXCR4 chemokine receptor surface expression, signaling and function in chronic lymphocytic leukemia.Leukemia · 2016Article
- Natural IgM is produced by CD5- plasma cells that occupy a distinct survival niche in bone marrow.Journal of immunology (Baltimore, Md. : 1950) · 2015Article
- Article
- Article
- Roles for BLyS family members in meeting the distinct homeostatic demands of innate and adaptive B cells.Frontiers in immunology · 2013Article
- Selection of individual VH genes occurs at the pro-B to pre-B cell transition.Journal of immunology (Baltimore, Md. : 1950) · 2011Article
- Progenitor cell origin plays a role in fate choices of mature B cells.Journal of immunology (Baltimore, Md. : 1950) · 2010Article
- Article
- Identification of an anti-idiotypic antibody that defines a B-cell subset(s) producing xenoantibodies in primates.Immunology · 2008Article
- Inherent specificities in natural antibodies: a key to immune defense against pathogen invasion.Springer seminars in immunopathology · 2005Review
- Molecular characterization of a panel of murine monoclonal antibodies specific for the SARS-coronavirus.Molecular immunology · 2005Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Antibodies to phosphatidylcholine (PtC), a common constituent of mammalian and bacterial cell membranes, represent a large proportion of the natural antibody repertoire in mice. Previous studies of several mouse strains (e.g., C57BL/6) have shown that anti-PtC antibodies are mainly encoded by the VH11 and VH12 immunoglobulin heavy chain variable region gene families. We show here, however, that VH11 and VH12 encode only a small proportion of the anti-PtC antibodies in BALB/c mice. Instead, VHQ52-encoded antibodies predominate in this strain. In addition, two-thirds of the cells expressing VHQ52 family genes use a single gene (which, interestingly, has been previously shown to predominate in the anti-oxazolone response). We also show here that in anti-PtC antibodies from all strains, the distinctive antigen-binding sites associated with VHQ52 differ substantially from those associated with VH11 and VH12. That is, VHQ52-containing transcripts preferentially use the joining region JH4 rather than JH1 and exhibit more diverse complementarity-determining region 3 (CDR3) junctions with more N-region nucleotide additions at the gene segment junctions. Thus, the VH gene family that predominates in the anti-PtC repertoire differs among mouse strains, whereas the distinctive VHDJH rearrangements (CDR3, JH) associated with each VH gene family are similar in all strains. We discuss these findings in the context of a recent hypothesis suggesting that CDR3 structure, independent of VH framework, is sufficient to define the specificity of an antibody.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.