ArticleProceedings of the National Academy of Sciences of the United States of America1999
Ligand-dependent activation of transcription in vitro by retinoic acid receptor alpha/retinoid X receptor alpha heterodimers that mimics transactivation by retinoids in vivo.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 50 citations in OpenAlex.
- Genome-wide chromatin profiling reveals a nonlimiting role for RXR in macrophage-like cells stimulated with multiple nuclear receptor agonists.The Journal of biological chemistry · 2026Article
- General molecular biology and architecture of nuclear receptors.Current topics in medicinal chemistry · 2012Review
- The target of the NSD family of histone lysine methyltransferases depends on the nature of the substrate.The Journal of biological chemistry · 2009Article
- p53 sumoylation: mechanistic insights from reconstitution studies.Epigenetics · 2009Article
- In vitro transcription system delineates the distinct roles of the coactivators pCAF and p300 during MyoD/E47-dependent transactivation.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- In vivo activation of PPAR target genes by RXR homodimers.The EMBO journal · 2004Article
- Sequential recruitment of steroid receptor coactivator-1 (SRC-1) and p300 enhances progesterone receptor-dependent initiation and reinitiation of transcription from chromatin.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- The novel transcription factor e(y)2 interacts with TAF(II)40 and potentiates transcription activation on chromatin templates.Molecular and cellular biology · 2001Article
- Review
- Cell-type-specific regulation of the retinoic acid receptor mediated by the orphan nuclear receptor TLX.Molecular and cellular biology · 2000Article
- Structure and dynamic properties of a glucocorticoid receptor-induced chromatin transition.Molecular and cellular biology · 2000Article
- Crystal structure of the human RXRalpha ligand-binding domain bound to its natural ligand: 9-cis retinoic acid.The EMBO journal · 2000Article
- p300 requires its histone acetyltransferase activity and SRC-1 interaction domain to facilitate thyroid hormone receptor activation in chromatin.Molecular and cellular biology · 2000Article
- Steroid receptor coactivator-1 (SRC-1) enhances ligand-dependent and receptor-dependent cell-free transcription of chromatin.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- A new widely distributed nuclear protein involved in the regulation of transcription catalyzed by RNA polymerase II.Doklady biochemistry : proceedings of the Academy of Sciences of the USSR, Biochemistry sectionArticle
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5 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
All-trans and 9-cis retinoic acids (RA) signals are transduced by retinoic acid receptor/retinoid X receptor (RAR/RXR) heterodimers that act as functional units controlling the transcription of RA-responsive genes. With the aim of elucidating the underlying molecular mechanisms, we have developed an in vitro transcription system using a chromatin template made up of a minimal promoter and a direct repeat with 5-spacing-based RA response element. RARalpha and RXRalpha were expressed in and purified from baculovirus-infected Sf9 cells, and transcription was carried out by using naked DNA or chromatin templates. Transcription from naked templates was not affected by the presence of RA and/or RAR/RXR heterodimers. In contrast, very little transcription occurred from chromatin templates in the absence of RA or RAR/RXR heterodimers whereas their addition resulted in a dosage-dependent stimulation of transcription that never exceeded that occurring on naked DNA templates. Most importantly, the addition of synthetic agonistic or antagonistic retinoids to the chromatin transcription system mimicked their stimulatory or inhibitory action in vivo, and activation by a RXR-specific retinoid was subordinated to the binding of an agonist ligand to the RAR partner. Moreover, the addition of the p300 coactivator generated a synergistic enhancement of transcription. Thus, the dissection of this transcription system ultimately should lead to the elucidation of the molecular mechanisms by which RAR/RXR heterodimers control transcription in a ligand-dependent manner.
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