ArticleProceedings of the National Academy of Sciences of the United States of America1999
An Escherichia coli strain with all chromosomal rRNA operons inactivated: complete exchange of rRNA genes between bacteria.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 123 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
123 citing papers in PubMed, 269 citations in OpenAlex.
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- Structural adaptations for enhanced translation kinetics in evolved ribosomes.bioRxiv : the preprint server for biology · 2026Article
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- Alternate conformational trajectories in ribosome translocation.PLoS computational biology · 2024Article
- Structural basis of Cfr-mediated antimicrobial resistance and mechanisms to evade it.Nature chemical biology · 2024Article
- Review
- Roles of the leader-trailer helix and antitermination complex in biogenesis of the 30S ribosomal subunit.Nucleic acids research · 2023Article
- Rare ribosomal RNA sequences from archaea stabilize the bacterial ribosome.Nucleic acids research · 2023Article
- Community science designed ribosomes with beneficial phenotypes.Nature communications · 2023Article
- Incompatibility and Interchangeability in Molecular Evolution.Genome biology and evolution · 2023Review
- Three-dimensional structure-guided evolution of a ribosome with tethered subunits.Nature chemical biology · 2022Article
- Regulatory perturbations of ribosome allocation in bacteria reshape the growth proteome with a trade-off in adaptation capacity.iScience · 2022Article
- Translational demand is not a major source of plasmid-associated fitness costs.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2022Article
- Targeted editing and evolution of engineered ribosomes in vivo by filtered editing.Nature communications · 2022Article
- A Survey of Spontaneous Antibiotic-Resistant Mutants of the Halophilic, Thermophilic BacteriumAntibiotics (Basel, Switzerland) · 2021Article
- Directed evolution of rRNA improves translation kinetics and recombinant protein yield.Nature communications · 2021Article
- Unexpected Discovery of Hypermutator Phenotype Sounds the Alarm for Quality Control Strains.Genome biology and evolution · 2021Article
- Insights into synthesis and function of KsgA/Dim1-dependent rRNA modifications in archaea.Nucleic acids research · 2021Article
63 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors at 2 institutions in 1 country.
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Abstract
Current global phylogenies are built predominantly on rRNA sequences. However, an experimental system for studying the evolution of rRNA is not readily available, mainly because the rRNA genes are highly repeated in most experimental organisms. We have constructed an Escherichia coli strain in which all seven chromosomal rRNA operons are inactivated by deletions spanning the 16S and 23S coding regions. A single E. coli rRNA operon carried by a multicopy plasmid supplies 16S and 23S rRNA to the cell. By using this strain we have succeeded in creating microorganisms that contain only a foreign rRNA operon derived from either Salmonella typhimurium or Proteus vulgaris, microorganisms that have diverged from E. coli about 120-350 million years ago. We also were able to replace the E. coli rRNA operon with an E. coli/yeast hybrid one in which the GTPase center of E. coli 23S rRNA had been substituted by the corresponding domain from Saccharomyces cerevisiae. These results suggest that, contrary to common belief, coevolution of rRNA with many other components in the translational machinery may not completely preclude the horizontal transfer of rRNA genes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.