ArticleProceedings of the National Academy of Sciences of the United States of America1999
Inhibitory sites in enzymes: zinc removal and reactivation by thionein.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 84 papers.
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Who cites it
84 citing papers in PubMed, 390 citations in OpenAlex.
- Dual-mode NRSC advances · 2026Article
- The human zinc-binding cysteine proteome.Cell · 2025Article
- Zinc-based Nanomaterials in Cancer Therapy: Mechanisms, Applications, and Future Directions.Theranostics · 2025Review
- Zinc Deficiency and Zinc Supplementation in Allergic Diseases.Biomolecules · 2024Review
- The Implications of Insufficient Zinc on the Generation of Oxidative Stress Leading to Decreased Oocyte Quality.Reproductive sciences (Thousand Oaks, Calif.) · 2023Review
- From methodological limitations to the function of metallothioneins - a guide to approaches for determining weak, moderate, and tight affinity zinc sites.Metallomics : integrated biometal science · 2023Article
- Comparison of Three Low-Molecular-Weight Fluorescent Probes for Measuring Free Zinc Levels in Cultured Mammary Cells.Nutrients · 2023Article
- Article
- Proton-Pump Inhibitors Suppress T Cell Response by Shifting Intracellular Zinc Distribution.International journal of molecular sciences · 2023Article
- A quinazolin-based Schiff-base chemosensor for colorimetric detection of NiRSC advances · 2022Article
- Neuronal signalling of zinc: from detection and modulation to function.Open biology · 2022Review
- Regulation of zinc-dependent enzymes by metal carrier proteins.Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine · 2022Review
- Divalent Metal Uptake and the Role of ZIP8 in Host Defense Against Pathogens.Frontiers in cell and developmental biology · 2022Review
- Lysosomal ZnCell reports · 2021Article
- The Enigmatic Metallothioneins: A Case of Upward-Looking Research.International journal of molecular sciences · 2021Article
- Antioxidant Defenses in the Human Eye: A Focus on Metallothioneins.Antioxidants (Basel, Switzerland) · 2021Review
- Article
- Chemistry and Biochemistry of Sulfur Natural Compounds: Key Intermediates of Metabolism and Redox Biology.Oxidative medicine and cellular longevity · 2020Review
- Procaspase-3 Overexpression in Cancer: A Paradoxical Observation with Therapeutic Potential.ACS chemical biology · 2019Review
- Multiple Mechanisms of Zinc-Mediated Inhibition for the Apoptotic Caspases-3, -6, -7, and -8.ACS chemical biology · 2018Article
24 more citing papers are in PubMed but not listed here.
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4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Thionein (T) has not been isolated previously from biological material. However, it is generated transiently in situ by removal of zinc from metallothionein under oxidoreductive conditions, particularly in the presence of selenium compounds. T very rapidly activates a group of enzymes in which zinc is bound at an inhibitory site. The reaction is selective, as is apparent from the fact that T does not remove zinc from the catalytic sites of zinc metalloenzymes. T instantaneously reverses the zinc inhibition with a stoichiometry commensurate with its known capacity to bind seven zinc atoms in the form of clusters in metallothionein. The zinc inhibition is much more pronounced than was previously reported, with dissociation constants in the low nanomolar range. Thus, T is an effective, endogenous chelating agent, suggesting the existence of a hitherto unknown and unrecognized biological regulatory system. T removes the metal from an inhibitory zinc-specific enzymatic site with a resultant marked increase of activity. The potential significance of this system is supported by the demonstration of its operations in enzymes involved in glycolysis and signal transduction.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.