ArticleOncogene1999
Nuclear and nucleolar targeting of human ribosomal protein S25: common features shared with HIV-1 regulatory proteins.
Article in Oncogene, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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Who cites it
18 citing papers in PubMed, 44 citations in OpenAlex.
- Bioinformatics and experimental validation identify biomarkers for diagnosing Alzheimer's disease.Frontiers in aging neuroscience · 2025Article
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- A memory of eS25 loss drives resistance phenotypes.Nucleic acids research · 2020Article
- Identification and fine mapping of nuclear and nucleolar localization signals within the human ribosomal protein S17.PloS one · 2015Article
- Elucidation of motifs in ribosomal protein S9 that mediate its nucleolar localization and binding to NPM1/nucleophosmin.PloS one · 2012Article
- PNRC accumulates in the nucleolus by interaction with B23/nucleophosmin via its nucleolar localization sequence.Biochimica et biophysica acta · 2011Article
- The nucleolus and viral infection.Virologica Sinica · 2010Review
- cDNA, genomic sequence cloning and overexpression of ribosomal protein S25 gene (RPS25) from the Giant Panda.Molecular biology reports · 2009Article
- Clusters of basic amino acids contribute to RNA binding and nucleolar localization of ribosomal protein L22.PloS one · 2009Article
- RNA viruses: hijacking the dynamic nucleolus.Nature reviews. Microbiology · 2007Review
- Intracellular localization of the severe acute respiratory syndrome coronavirus nucleocapsid protein: absence of nucleolar accumulation during infection and after expression as a recombinant protein in vero cells.Journal of virology · 2005Article
- The moving parts of the nucleolus.Histochemistry and cell biology · 2005Review
- Nuclear localization of the adenovirus E4orf4 protein is mediated through an arginine-rich motif and correlates with cell death.Oncogene · 2004Article
- Article
- Review
- Identification of nucleolus localization signal of betanodavirus GGNNV protein alpha.Virology · 2003Article
- Article
- Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
The nuclear and nucleolar targeting properties of human ribosomal protein S25 (RPS25) were analysed by the expression of epitope-tagged RPS25 cDNAs in Cos-1 cells. The tagged RPS25 was localized to the cell nucleus, with a strong predominance in the nucleolus. At the amino terminus of RPS25, two stretches of highly basic residues juxtapose. This configuration shares common features with the nucleolar targeting signals (NOS) of lentiviral RNA-binding transactivators, including human immunodeficiency viruses' (HIV) Rev proteins. Deletion and site-directed mutational analyses demonstrated that the first NOS-like stretch is dispensable for both nuclear and nucleolar localization of RPS25, and that the nuclear targeting signal is located within the second NOS-like stretch. It has also been suggested that a set of continuous basic residues and the total number of basic residues should be required for nucleolar targeting. Signal-mediated nuclear/nucleolar targeting was further characterized by the construction and expression of a variety of chimeric constructs, utilizing three different backbones with RPS25 cDNA fragments. Immunofluorescence analyses demonstrated a 17 residue peptide of RPS25 as a potential nuclear/nucleolar targeting signal. The identified peptide signal may belong to a putative subclass of NOS, characterized by compact structure, together with lentiviral RNA-binding transactivators.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.