ArticleThe Journal of physiology1999
Contribution of delayed rectifier potassium currents to the electrical activity of murine colonic smooth muscle.
Article in The Journal of physiology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- Beyond the Alpha Subunit: Pharmacological Modulation of Kv4.2 Channels by Ancillary Proteins.Cells · 2026Review
- Organic Synthesis of New Secosteroids from Fucosterol, Its Intestinal Absorption by Caco-2 Cells, and Simulation of the Biological Activities of Vitamin D.Marine drugs · 2023Article
- The Role of Ion Channels in Functional Gastrointestinal Disorders (FGID): Evidence of Channelopathies and Potential Avenues for Future Research and Therapeutic Targets.International journal of molecular sciences · 2023Review
- Characterization of the A-type potassium current in murine gastric fundus smooth muscles.American journal of physiology. Cell physiology · 2021Article
- Arecoline hydrobromide enhances jejunum smooth muscle contractility via voltage-dependent potassium channels in W/Wv mice.Physiological research · 2021Article
- Adiponectin Exerts Peripheral Inhibitory Effects on the Mouse Gastric Smooth Muscle through the AMPK Pathway.International journal of molecular sciences · 2020Article
- TRPM8 Channel Activation Reduces the Spontaneous Contractions in Human Distal Colon.International journal of molecular sciences · 2020Article
- Molecular and functional characterization of inwardly rectifying KThe Journal of physiology · 2018Article
- Inhibitory effects of SKF96365 on the activities of K(+) channels in mouse small intestinal smooth muscle cells.The Journal of veterinary medical science · 2016Article
- The role of K⁺ conductances in regulating membrane excitability in human gastric corpus smooth muscle.American journal of physiology. Gastrointestinal and liver physiology · 2015Article
- Modulation of Closed-State Inactivation in Kv2.1/Kv6.4 Heterotetramers as Mechanism for 4-AP Induced Potentiation.PloS one · 2015Article
- Voltage dependent potassium channel remodeling in murine intestinal smooth muscle hypertrophy induced by partial obstruction.PloS one · 2014Article
- Effect of mosapride on Kv4.3 potassium channels expressed in CHO cells.Naunyn-Schmiedeberg's archives of pharmacology · 2013Article
- Ionic conductances regulating the excitability of colonic smooth muscles.Neurogastroenterology and motility · 2012Review
- Kv4.2 knockout mice have hippocampal-dependent learning and memory deficits.Learning & memory (Cold Spring Harbor, N.Y.) · 2012Article
- Regulation of gastrointestinal motility by Ca2+/calmodulin-stimulated protein kinase II.Archives of biochemistry and biophysics · 2011Review
- Phospholipase C-independent effects of 3M3FBS in murine colon.European journal of pharmacology · 2010Article
- Regulation of smooth muscle excitation and contraction.Neurogastroenterology and motility · 2008Review
- Effects of female steroid hormones on A-type K+ currents in murine colon.The Journal of physiology · 2006Article
- A role for voltage-gated, but not Ca2+-activated, K+ channels in regulating spontaneous contractile activity in myometrium from virgin and pregnant rats.British journal of pharmacology · 2006Article
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8 authors.
Funding
Abstract
1. We used intracellular microelectrodes to record the membrane potential (Vm) of intact murine colonic smooth muscle. Electrical activity consisted of spike complexes separated by quiescent periods (Vm approximately -60 mV). The spike complexes consisted of about a dozen action potentials of approximately 30 mV amplitude. Tetraethylammonium (TEA, 1-10 mM) had little effect on the quiescent periods but increased the amplitude of the action potential spikes. 4-Aminopyridine (4-AP, >= 5 mM) caused continuous spiking. 2. Voltage clamp of isolated myocytes identified delayed rectifier K+ currents that activated rapidly (time to half-maximum current, 11.5 ms at 0 mV) and inactivated in two phases (tauf = 96 ms, taus = 1.5 s at 0 mV). The half-activation voltage of the permeability was -27 mV, with significant activation at -50 mV. 3. TEA (10 mM) reduced the outward current at potentials positive to 0 mV. 4-AP (5 mM) reduced the early current but increased outward current at later times (100-500 ms) consistent with block of resting channels relieved by depolarization. 4-AP inhibited outward current at potentials negative to -20 mV, potentials where TEA had no effect. 4. Qualitative PCR amplification of mRNA identified transcripts encoding delayed rectifier K+ channel subunits Kv1.6, Kv4.1, Kv4.2, Kv4.3 and the Kvbeta1.1 subunit in murine colon myocytes. mRNA encoding Kv 1.4 was not detected. 5. We find that TEA-sensitive delayed rectifier currents are important determinants of action potential amplitude but not rhythmicity. Delayed rectifier currents sensitive to 4-AP are important determinants of rhythmicity but not action potential amplitude.
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