ArticleThe Journal of physiology1999
Glucose-receptive neurones in the rat ventromedial hypothalamus express KATP channels composed of Kir6.1 and SUR1 subunits.
Article in The Journal of physiology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed, 1 synthesis or guideline pooled it, 90 citations in OpenAlex.
- A K(ATP) channel gene effect on sleep duration: from genome-wide association studies to function in Drosophila.Molecular psychiatry · 2013Pooled it
- The effect of modafinil on counter-regulatory and cognitive responses to hypoglycaemia.Diabetologia · 2004Trial
- AMPK-mediated potentiation of GABAergic signalling drives hypoglycaemia-provoked spike-wave seizures.Brain : a journal of neurology · 2022Article
- Loss of SUR1 subtype KBehavioural brain research · 2021Article
- Brain Glucose-Sensing Mechanism and Energy Homeostasis.Molecular neurobiology · 2019Review
- Modulation of Excitability of Stellate Neurons in the Ventral Cochlear Nucleus of Mice by ATP-Sensitive Potassium Channels.The Journal of membrane biology · 2018Article
- Glucose Enhances Basal or Melanocortin-Induced cAMP-Response Element Activity in Hypothalamic Cells.Molecular endocrinology (Baltimore, Md.) · 2016Article
- Neural pathways that control the glucose counterregulatory response.Frontiers in neuroscience · 2014Review
- Review of the neuroanatomic landscape implicated in glucose sensing and regulation of nutrient signaling: immunophenotypic localization of diabetes gene Tcf7l2 in the developing murine brain.Journal of chemical neuroanatomy · 2012Review
- Mouse hypothalamic GT1-7 cells demonstrate AMPK-dependent intrinsic glucose-sensing behaviour.Diabetologia · 2012Article
- Characterization of an ATP-sensitive K(+) channel in rat carotid body glomus cells.Respiratory physiology & neurobiology · 2011Article
- Sensing the fuels: glucose and lipid signaling in the CNS controlling energy homeostasis.Cellular and molecular life sciences : CMLS · 2010Review
- ATP-sensitive potassium channel-mediated lactate effect on orexin neurons: implications for brain energetics during arousal.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2010Article
- Glucose sensing neurons in the ventromedial hypothalamus.Sensors (Basel, Switzerland) · 2010Review
- ATP-sensitive potassium currents reduce the PGE2-mediated enhancement of excitability in adult rat sensory neurons.Brain research · 2007Article
- Article
- The aminoguanidine carboxylate BVT.12777 activates ATP-sensitive K+ channels in the rat insulinoma cell line, CRI-G1.BMC pharmacology · 2004Article
- Modulation of the excitability of cholinergic basal forebrain neurones by KATP channels.The Journal of physiology · 2004Article
- Sulphonylurea action revisited: the post-cloning era.Diabetologia · 2003Review
- Orexins induce increased excitability and synchronisation of rat sympathetic preganglionic neurones.The Journal of physiology · 2003Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
1. Patch-clamp recordings were made from rat ventromedial hypothalamic neurones in slices of brain tissue in vitro. In cell-attached recordings, removal of extracellular glucose or metabolic inhibition with sodium azide reduced the firing rate of a subpopulation of cells through the activation of a 65 pS channel that was blocked by the sulphonylureas tolbutamide and glibenclamide. 2. In whole-cell patch-clamp recordings, in the absence of ATP in the electrode solution, glucose-receptive neurones gradually hyperpolarized due to the induction of an outward current at -60 mV. This outward current and the resultant hyperpolarization were blocked by the sulphonylureas tolbutamide and glibenclamide. 3. In recordings where the electrode solution contained 4 mM ATP, this outward current was not observed. Under these conditions, 500 microM diazoxide was found to induce an outward current that was blocked by tolbutamide. 4. In cell-attached recordings diazoxide and the active fragment of leptin (leptin 22-56) reduced the firing rate of glucose-receptive neurones by the activation of a channel with similar properties to that induced by removal of extracellular glucose. 5. Reverse transcription followed by the polymerase chain reaction using cytoplasm from single glucose-receptive neurones demonstrated the expression of the ATP-sensitive potassium (KATP) channel subunits Kir6.1 and SUR1 but not Kir6.2 or SUR2. 6. It is concluded that glucose-receptive neurones within the rat ventromedial hypothalamus exhibit a KATP channel current with pharmacological and molecular properties similar to those reported in other tissues.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.