ArticleThe Journal of physiology1999
Depolarization-evoked Ca2+ release in a non-excitable cell, the rat megakaryocyte.
Article in The Journal of physiology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 49 citations in OpenAlex.
- Electrical Stimulation in Cartilage Tissue Engineering.Bioengineering (Basel, Switzerland) · 2023Review
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- ATP inhibits Ins(1,4,5)P3-evoked Ca2+ release in smooth muscle via P2Y1 receptors.Journal of cell science · 2012Article
- SNARE-dependent glutamate release in megakaryocytes.Experimental hematology · 2010Article
- Direct voltage control of endogenous lysophosphatidic acid G-protein-coupled receptors in Xenopus oocytes.The Journal of physiology · 2010Article
- Acetylsalicylic acid enhances purinergic receptor-mediated outward currents in rat megakaryocytes.American journal of physiology. Cell physiology · 2010Article
- Novel consequences of voltage-dependence to G-protein-coupled P2Y1 receptors.British journal of pharmacology · 2008Article
- Kit mutants and gastrointestinal physiology.The Journal of physiology · 2007Review
- Modulation of Gq-protein-coupled inositol trisphosphate and Ca2+ signaling by the membrane potential.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2006Article
- Role of voltage-dependent modulation of store Ca2+ release in synchronization of Ca2+ oscillations.Biophysical journal · 2006Article
- The interpretation of current-clamp recordings in the cell-attached patch-clamp configuration.Biophysical journal · 2005Article
- Sensitivity limits for voltage control of P2Y receptor-evoked Ca2+ mobilization in the rat megakaryocyte.The Journal of physiology · 2004Article
- Pacing of interstitial cells of Cajal in the murine gastric antrum: neurally mediated and direct stimulation.The Journal of physiology · 2003Article
- Properties of the demarcation membrane system in living rat megakaryocytes.Biophysical journal · 2003Article
- Ca2+ phase waves: a basis for cellular pacemaking and long-range synchronicity in the guinea-pig gastric pylorus.The Journal of physiology · 2003Article
- A theoretical model of slow wave regulation using voltage-dependent synthesis of inositol 1,4,5-trisphosphate.Biophysical journal · 2002Article
- A study of P2X1 receptor function in murine megakaryocytes and human platelets reveals synergy with P2Y receptors.British journal of pharmacology · 2002Article
- Depolarisation-evoked Ca2+ waves in the non-excitable rat megakaryocyte.The Journal of physiology · 2001Article
- Distribution of pacemaker function through the tunica muscularis of the canine gastric antrum.The Journal of physiology · 2001Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
1. The effect of membrane potential on [Ca2+]i in rat megakaryocytes was studied using simultaneous whole-cell patch clamp and fura-2 fluorescence recordings. 2. Depolarization from -75 to 0 mV had no effect on [Ca2+]i in unstimulated cells, but evoked one or more spikes of Ca2+ increase (peak increase: 714 +/- 95 nM) during activation of metabotropic purinoceptors by 1 microM ADP. 3. The depolarization-evoked Ca2+ increase was present in Ca2+-free medium and also following removal of Na+. Thus depolarization mobilizes Ca2+ from an intracellular store without a requirement for altered Na+-Ca2+ exchange activity. 4. Intracellular dialysis with heparin blocked the depolarization-evoked Ca2+ increase, indicating a role for functional IP3 receptors. 5. Under current clamp, ADP caused the membrane potential to fluctuate between -43 +/- 1 and -76 +/- 1 mV. Under voltage clamp, depolarization from -75 to -45 mV evoked a transient [Ca2+]i increase (398 +/- 91 nM) during exposure to ADP. 6. We conclude that during stimulation of metabotropic purinoceptors, membrane depolarization over the physiological range can stimulate Ca2+ release from intracellular stores in the rat megakaryocyte, a non-excitable cell type. This may represent an important mechanism by which electrogenic influences can control patterns of [Ca2+]i increase.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.