ArticleThe Journal of physiology1999
Pulsatile shear stress leads to DNA fragmentation in human SH-SY5Y neuroblastoma cell line.
Article in The Journal of physiology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 52 citations in OpenAlex.
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- Use of dual-flow bioreactor to develop a simplified model of nervous-cardiovascular systems crosstalk: A preliminary assessment.PloS one · 2020Article
- Optic disc cupping characteristics of normal pressure hydrocephalus patients with normal-tension glaucoma.Scientific reports · 2019Article
- Acoustofluidic Rotational Manipulation of Cells and Organisms Using Oscillating Solid Structures.Small (Weinheim an der Bergstrasse, Germany) · 2016Article
- Ruthenium complexes as NO donors for vascular relaxation induction.Molecules (Basel, Switzerland) · 2014Review
- Thermal expansion of vitrified blood vessels permeated with DP6 and synthetic ice modulators.Cryobiology · 2014Article
- Eye-specific IOP-induced displacements and deformations of human lamina cribrosa.Investigative ophthalmology & visual science · 2014Article
- Oxidative stress, DNA damage and repair in heart failure patients after implantation of continuous flow left ventricular assist devices.International journal of medical sciences · 2013Article
- Substratum compliance regulates human trabecular meshwork cell behaviors and response to latrunculin B.Investigative ophthalmology & visual science · 2011Article
- Viscoelastic Modeling of Optic Nerve Head Biomechanics: Effects of Intraocular and Cerebrospinal Fluid Pressure.Biocybernetics and biomedical engineeringArticle
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
1. Using an in vitro model of shear stress-induced cell injury we demonstrate that application of shear to differentiated human SH-SY5Y cells leads to cell death characterized by DNA fragmentation. Controlled shear stress was applied to cells via a modified cone and plate viscometer. 2. We show that pulsatile shear stress leads to DNA fragmentation, as determined via flow cytometry of fluorescein-12-dUTP nick-end labelled cells, in 45 +/- 4 % of cells. No lactate dehydrogenase (LDH) release was observed immediately after injury; however, 24 h after injury significant LDH release was observed. 3. Nitric oxide production by cells subjected to pulsatile shear increased two- to threefold over that in unsheared control cells. 4. Inhibition of protein synthesis, nitric oxide production, Ca2+ entry into cells, and pertussis toxin-sensitive G protein activation attenuated the shear stress-induced cell injury. 5. Our results show for the first time that application of pulsatile shear stress to a neuron-like cell in vitro leads to nitric oxide-dependent cell death.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.