ArticleGenetics1999
Conversion of lacZ enhancer trap lines to GAL4 lines using targeted transposition in Drosophila melanogaster.
Article in Genetics, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 77 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
77 citing papers in PubMed.
- Neuronal loss of the pentose phosphate pathway in the living nervous system is causally linked to [NADPH] reduction and elevated oxidative stress.The Journal of physiology · 2026Article
- microRNA-184 distribution and consequences on glial septate junctions and the blood-brain barrier.PloS one · 2025Article
- Generation of LexA enhancer-trap lines in Drosophila by an international scholastic network.G3 (Bethesda, Md.) · 2023Article
- Glycolytically impaired Drosophila glial cells fuel neural metabolism via β-oxidation.Nature communications · 2023Article
- Extracellular matrix assembly stress initiates Drosophila central nervous system morphogenesis.Developmental cell · 2023Article
- Innexin-Mediated Adhesion between Glia Is Required for Axon Ensheathment in the Peripheral Nervous System.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2023Article
- Gliotransmission of D-serine promotes thirst-directed behaviors in Drosophila.Current biology : CB · 2022Article
- Brain inflammation triggers macrophage invasion across the blood-brain barrier inScience advances · 2021Article
- Oligodendrocytes Provide Antioxidant Defense Function for Neurons by Secreting Ferritin Heavy Chain.Cell metabolism · 2020Article
- Basigin Associates with Integrin in Order to Regulate Perineurial Glia andThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2020Article
- Activity-dependent visualization and control of neural circuits for courtship behavior in the flyProceedings of the National Academy of Sciences of the United States of America · 2019Article
- Article
- The glial sodium-potassium-2-chloride cotransporter is required for synaptic transmission in the Drosophila visual system.Scientific reports · 2019Article
- Expressional Profiling of Carpet Glia in the DevelopingFrontiers in neuroscience · 2019Article
- Downregulation of glutamic acid decarboxylase in Drosophila TDP-43-null brains provokes paralysis by affecting the organization of the neuromuscular synapses.Scientific reports · 2018Article
- Chaski, a novel Drosophila lactate/pyruvate transporter required in glia cells for survival under nutritional stress.Scientific reports · 2018Article
- Characterization of a morphogenetic furrow specific Gal4 driver in the developing Drosophila eye.PloS one · 2018Article
- The extracellular metalloprotease AdamTS-A anchors neural lineages in place within and preserves the architecture of the central nervous system.Development (Cambridge, England) · 2017Article
- Regulation of Drosophila hematopoietic sites by Activin-β from active sensory neurons.Nature communications · 2017Article
- Dynamic analysis of the mesenchymal-epithelial transition of blood-brain barrier forming glia inBiology open · 2017Article
17 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Since the development of the enhancer trap technique, many large libraries of nuclear localized lacZ P-element stocks have been generated. These lines can lend themselves to the molecular and biological characterization of new genes. However they are not as useful for the study of development of cellular morphologies. With the advent of the GAL4 expression system, enhancer traps have a far greater potential for utility in biological studies. Yet generation of GAL4 lines by standard random mobilization has been reported to have a low efficiency. To avoid this problem we have employed targeted transposition to generate glial-specific GAL4 lines for the study of glial cellular development. Targeted transposition is the precise exchange of one P element for another. We report the successful and complete replacement of two glial enhancer trap P[lacZ, ry+] elements with the P[GAL4, w+] element. The frequencies of transposition to the target loci were 1.3% and 0.4%. We have thus found it more efficient to generate GAL4 lines from preexisting P-element lines than to obtain tissue-specific expression of GAL4 by random P-element mobilization. It is likely that similar screens can be performed to convert many other P-element lines to the GAL4 system.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.