ArticleGenetics1999
Genetic analysis of viable Hsp90 alleles reveals a critical role in Drosophila spermatogenesis.
Article in Genetics, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 127 citations in OpenAlex.
- Organismal Roles of Hsp90.Biomolecules · 2023Review
- Review
- Hyperthermia increases HSP production in human PDMCs by stimulating ROS formation, p38 MAPK and Akt signaling, and increasing HSF1 activity.Stem cell research & therapy · 2022Article
- Article
- Mitochondrial-Y chromosome epistasis inProceedings. Biological sciences · 2020Article
- Article
- Ubiquitome analysis reveals the involvement of lysine ubiquitination in the spermatogenesis process of adult buffalo (Bubalus bubalis) testis.Bioscience reports · 2020Article
- What's Genetic Variation Got to Do with It? Starvation-Induced Self-Fertilization Enhances Survival in Paramecium.Genome biology and evolution · 2020Article
- AMolecular & cellular proteomics : MCP · 2017Article
- Hsp83 loss suppresses proteasomal activity resulting in an upregulation of caspase-dependent compensatory autophagy.Autophagy · 2017Article
- Heat shock protein 83 plays pleiotropic roles in embryogenesis, longevity, and fecundity of the pea aphid Acyrthosiphon pisum.Development genes and evolution · 2017Article
- The de-ubiquitylating enzyme DUBA is essential for spermatogenesis in Drosophila.Cell death and differentiation · 2016Article
- The Mechanism of Hsp90 ATPase Stimulation by Aha1.Scientific reports · 2016Article
- Article
- Characterizing heat shock protein 90 gene of Apolygus lucorum (Meyer-Dür) and its expression in response to different temperature and pesticide stresses.Cell stress & chaperones · 2014Article
- Kdm3a lysine demethylase is an Hsp90 client required for cytoskeletal rearrangements during spermatogenesis.Molecular biology of the cell · 2014Article
- Influence of the temperature and the genotype of the HSP90AA1 gene over sperm chromatin stability in Manchega Rams.PloS one · 2014Article
- Functional roles for redox genes in ethanol sensitivity in Drosophila.Functional & integrative genomics · 2012Article
- Hsp90 is important for fecundity, longevity, and buffering of cryptic deleterious variation in wild fly populations.BMC evolutionary biology · 2012Article
- Post-embryonic functions of HSP90 in Tribolium castaneum include the regulation of compound eye development.Development genes and evolution · 2011Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Hsp90 chaperone protein maintains the activities of a remarkable variety of signal transducers, but its most critical functions in the context of the whole organism are unknown. Point mutations of Hsp83 (the Drosophila Hsp90 gene) obtained in two different screens are lethal as homozygotes. We report that eight transheterozygous mutant combinations produce viable adults. All exhibit the same developmental defects: sterile males and sterile or weakly fertile females. We also report that scratch, a previously identified male-sterile mutation, is an allele of Hsp82 with a P-element insertion in the intron that reduces expression. Thus, it is a simple reduction in Hsp90 function, rather than possible altered functions in the point mutants, that leads to male sterility. As shown by light and electron microscopy, all stages of spermatogenesis involving microtubule function are affected, from early mitotic divisions to later stages of sperm maturation, individualization, and motility. Aberrant microtubules are prominent in yeast cells carrying mutations in HSP82 (the yeast Hsp90 gene), confirming that Hsp90 function is connected to microtubule dynamics and that this connection is highly conserved. A small fraction of Hsp90 copurifies with taxol-stabilized microtubule proteins in Drosophila embryo extracts, but Hsp90 does not remain associated with microtubules through repeated temperature-induced assembly and disassembly reactions. If the spermatogenesis phenotypes are due to defects in microtubule dynamics, we suggest these are indirect, reflecting a role for Hsp90 in maintaining critical signal transduction pathways and microtubule effectors, rather than a direct role in the assembly and disassembly of microtubules themselves.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.