ArticleApplied and environmental microbiology1999
Identification of Epichloë endophytes in planta by a microsatellite-based PCR fingerprinting assay with automated analysis.
Article in Applied and environmental microbiology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 129 citations in OpenAlex.
- Genetic Covariation Between the Vertically Transmitted Endophyte Epichloë canadensis and Its Host Canada Wildrye.Microbial ecology · 2023Article
- The Impact of Alkaloid-ProducingToxins · 2021Review
- Lolium perenne apoplast metabolomics for identification of novel metabolites produced by the symbiotic fungus Epichloë festucae.The New phytologist · 2020Article
- A Tale of Two Grass Species: Temperature Affects the Symbiosis of a MutualisticFrontiers in plant science · 2020Article
- Infection Rates and Alkaloid Patterns of Different Grass Species with SystemicApplied and environmental microbiology · 2019Article
- A homologue of the fungal tetraspanin Pls1 is required for Epichloë festucae expressorium formation and establishment of a mutualistic interaction with Lolium perenne.Molecular plant pathology · 2019Article
- Analysis of Epichloë festucae small secreted proteins in the interaction with Lolium perenne.PloS one · 2019Article
- Large Scale Screening ofFrontiers in plant science · 2019Article
- Repeat elements organise 3D genome structure and mediate transcription in the filamentous fungus Epichloë festucae.PLoS genetics · 2018Article
- Disruption of calcineurin catalytic subunit (cnaA) in Epichloë festucae induces symbiotic defects and intrahyphal hyphae formation.Molecular plant pathology · 2018Article
- Fungal Endophytes: Beyond Herbivore Management.Frontiers in microbiology · 2018Review
- Article
- An Epichloë festucae homologue of MOB3, a component of the STRIPAK complex, is required for the establishment of a mutualistic symbiotic interaction with Lolium perenne.Molecular plant pathology · 2016Article
- The endophytic symbiont Epichloë festucae establishes an epiphyllous net on the surface of Lolium perenne leaves by development of an expressorium, an appressorium-like leaf exit structure.The New phytologist · 2016Article
- Primers to amplify SNP markers in Epichloë canadensis (Clavicipitaceae).Applications in plant sciences · 2016Article
- cAMP Signaling Regulates Synchronised Growth of SymbioticFrontiers in plant science · 2016Article
- Advances in Research on Epichloë endophytes in Chinese Native Grasses.Frontiers in microbiology · 2016Review
- Genetic evidence for reproductive isolation among sympatric Epichloë endophytes as inferred from newly developed microsatellite markers.Microbial ecology · 2015Article
- Phylogenomics of asexual Epichloë fungal endophytes forming associations with perennial ryegrass.BMC evolutionary biology · 2015Article
- Disparate independent genetic events disrupt the secondary metabolism gene perA in certain symbiotic Epichloë species.Applied and environmental microbiology · 2015Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Epichloë endophytes are a group of filamentous fungi that include both sexual (Epichloë) and asexual (Neotyphodium) species. As a group they are genetically diverse and form both antagonistic and mutualistic associations with temperate grasses. We report here on the development of a microsatellite-based PCR system for fingerprinting this group of fungi with template isolated from either culture or infected plant material. M13mp19 partial genomic libraries were constructed for size-fractionated genomic DNA from two endophyte strains. These libraries were screened with a mixture of DIG-labeled dinucleotide and trinucleotide repeat probes. Positive clones were sequenced, and nine unique microsatellite loci were identified. An additional microsatellite was serendipitously identified in the 3' untranscribed region of the 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase gene from N. lolii Lp19. Primers were designed for each locus and a panel of endophytes, from different taxonomic groupings, was screened to determine the degree of polymorphism. On the basis of these results a multiplex assay was developed for strain identification with fluorescently labeled primers for five of these loci. Using this system the size of the products amplified can be precisely determined by automated analysis, and an allele profile for each strain can be readily generated. The assay was shown to resolve endophyte groupings to the level of known isozyme phenotype groupings. In a blind test the assay was used successfully to identify a set of endophytes in planta. A reference database of allele sizes has been established for the panel of endophytes examined, and this will be expanded as new strains are analyzed.
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