ArticleApplied and environmental microbiology1999
High-affinity methane oxidation by a soil enrichment culture containing a type II methanotroph.
Article in Applied and environmental microbiology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed.
- Physiological basis for atmospheric methane oxidation and methanotrophic growth on air.Nature communications · 2024Article
- Resource availability governs polyhydroxyalkanoate (PHA) accumulation and diversity of methanotrophic enrichments from wetlands.Frontiers in bioengineering and biotechnology · 2023Article
- Rainforest-to-pasture conversion stimulates soil methanogenesis across the Brazilian Amazon.The ISME journal · 2021Article
- Article
- Genomic and Physiological Properties of a Facultative Methane-Oxidizing Bacterial Strain ofMicroorganisms · 2020Article
- Methane oxidation and methylotroph population dynamics in groundwater mesocosms.Environmental microbiology · 2020Article
- Co-cultivation of the strictly anaerobic methanogen Methanosarcina barkeri with aerobic methanotrophs in an oxygen-limited membrane bioreactor.Applied microbiology and biotechnology · 2018Article
- The hunt for the most-wanted chemolithoautotrophic spookmicrobes.FEMS microbiology ecology · 2018Article
- Differentiated Mechanisms of Biochar Mitigating Straw-Induced Greenhouse Gas Emissions in Two Contrasting Paddy Soils.Frontiers in microbiology · 2018Article
- Enrichment culture and identification of endophytic methanotrophs isolated from peatland plants.Folia microbiologica · 2017Article
- Microbial and Isotopic Evidence for Methane Cycling in Hydrocarbon-Containing Groundwater from the Pennsylvania Region.Frontiers in microbiology · 2017Article
- Persistence of the dominant soil phylum Acidobacteria by trace gas scavenging.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Article
- Diversity and Habitat Preferences of Cultivated and Uncultivated Aerobic Methanotrophic Bacteria Evaluated Based on pmoA as Molecular Marker.Frontiers in microbiology · 2015Review
- Effects of Long-Term CO2 Enrichment on Soil-Atmosphere CH4 Fluxes and the Spatial Micro-Distribution of Methanotrophic Bacteria.PloS one · 2015Article
- Microbial CH(4) and N(2)O Consumption in Acidic Wetlands.Frontiers in microbiology · 2012Article
- Different atmospheric methane-oxidizing communities in European beech and Norway spruce soils.Applied and environmental microbiology · 2010Article
- Molecular ecology techniques for the study of aerobic methanotrophs.Applied and environmental microbiology · 2008Review
- Effect of afforestation and reforestation of pastures on the activity and population dynamics of methanotrophic bacteria.Applied and environmental microbiology · 2007Article
- Mixed pollutant degradation by Methylosinus trichosporium OB3b expressing either soluble or particulate methane monooxygenase: can the tortoise beat the hare?Applied and environmental microbiology · 2006Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Methanotrophic bacteria in an organic soil were enriched on gaseous mixing ratios of <275 parts per million of volume (ppmv) of methane (CH4). After 4 years of growth and periodic dilution (>10(20) times the initial soil inoculum), a mixed culture was obtained which displayed an apparent half-saturation constant [Km(app)] for CH4 of 56 to 186 nM (40 to 132 ppmv). This value was the same as that measured in the soil itself and about 1 order of magnitude lower than reported values for pure cultures of methane oxidizers. However, the Km(app) increased when the culture was transferred to higher mixing ratios of CH4 (1,000 ppmv, or 1%). Denaturing gradient gel electrophoresis of the enrichment grown on <275 ppmv of CH4 revealed a single gene product of pmoA, which codes for a subunit of particulate methane monooxygenase. This suggested that only one methanotroph species was present. This organism was isolated from a sample of the enrichment culture grown on 1% CH4 and phylogenetically positioned based on its 16S rRNA, pmoA, and mxaF gene sequences as a type II strain of the Methylocystis/Methylosinus group. A coculture of this strain with a Variovorax sp., when grown on <275 ppmv of CH4, had a Km(app) (129 to 188 nM) similar to that of the initial enrichment culture. The data suggest that the affinity of methanotrophic bacteria for CH4 varies with growth conditions and that the oxidation of atmospheric CH4 observed in this soil is carried out by type II methanotrophic bacteria which are similar to characterized species.
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