ArticleJournal of bacteriology1999
The PalkBFGHJKL promoter is under carbon catabolite repression control in Pseudomonas oleovorans but not in Escherichia coli alk+ recombinants.
Article in Journal of bacteriology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 58 citations in OpenAlex.
- Review
- A novel alkane monooxygenase (PeerJ · 2022Article
- Synthetic Biology Approaches to Hydrocarbon Biosensors: A Review.Frontiers in bioengineering and biotechnology · 2021Review
- Maximizing Biocatalytic Cyclohexane Hydroxylation by Modulating Cytochrome P450 Monooxygenase Expression inFrontiers in bioengineering and biotechnology · 2020Article
- Specific Effect of Trace Metals on Marine Heterotrophic Microbial Activity and Diversity: Key Role of Iron and Zinc and Hydrocarbon-Degrading Bacteria.Frontiers in microbiology · 2018Article
- The Interaction between Plants and Bacteria in the Remediation of Petroleum Hydrocarbons: An Environmental Perspective.Frontiers in microbiology · 2016Review
- Making variability less variable: matching expression system and host for oxygenase-based biotransformations.Journal of industrial microbiology & biotechnology · 2015Article
- Variability in subpopulation formation propagates into biocatalytic variability of engineered Pseudomonas putida strains.Frontiers in microbiology · 2015Article
- Metabolic engineering of Pseudomonas sp. strain VLB120 as platform biocatalyst for the production of isobutyric acid and other secondary metabolites.Microbial cell factories · 2014Article
- Cell physiology rather than enzyme kinetics can determine the efficiency of cytochrome P450-catalyzed C-H-oxyfunctionalization.Journal of industrial microbiology & biotechnology · 2011Article
- Positively regulated bacterial expression systems.Microbial biotechnology · 2009Review
- NADH availability limits asymmetric biocatalytic epoxidation in a growing recombinant Escherichia coli strain.Applied and environmental microbiology · 2008Article
- Product repression of alkane monooxygenase expression in Pseudomonas butanovora.Journal of bacteriology · 2006Article
- Specificity at the end of the tunnel: understanding substrate length discrimination by the AlkB alkane hydroxylase.Journal of bacteriology · 2005Article
- Expression of the Pseudomonas putida OCT plasmid alkane degradation pathway is modulated by two different global control signals: evidence from continuous cultures.Journal of bacteriology · 2003Article
- Differential expression of the components of the two alkane hydroxylases from Pseudomonas aeruginosa.Journal of bacteriology · 2003Article
- Control of inducer accumulation plays a key role in succinate-mediated catabolite repression in Sinorhizobium meliloti.Journal of bacteriology · 2002Article
- Inactivation of cytochrome o ubiquinol oxidase relieves catabolic repression of the Pseudomonas putida GPo1 alkane degradation pathway.Journal of bacteriology · 2002Article
- Characterization and application of xylene monooxygenase for multistep biocatalysis.Applied and environmental microbiology · 2002Article
- Role of the crc gene in catabolic repression of the Pseudomonas putida GPo1 alkane degradation pathway.Journal of bacteriology · 2001Article
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The alk genes are located on the OCT plasmid of Pseudomonas oleovorans and encode an inducible pathway for the utilization of n-alkanes as carbon and energy sources. We have investigated the influence of alternative carbon sources on the induction of this pathway in P. oleovorans and Escherichia coli alk+ recombinants. In doing so, we confirmed earlier reports that induction of alkane hydroxylase activity in pseudomonads is subject to carbon catabolite repression. Specifically, synthesis of the monooxygenase component AlkB is repressed at the transcriptional level. The alk genes have been cloned into plasmid pGEc47, which has a copy number of about 5 to 10 per cell in both E. coli and pseudomonads. Pseudomonas putida GPo12 is a P. oleovorans derivative cured of the OCT plasmid. Upon introduction of pGEc47 in this strain, carbon catabolite repression of alkane hydroxylase activity was reduced significantly. In cultures of recombinant E. coli HB101 and W3110 carrying pGEc47, induction of AlkB and transcription of the alkB gene were no longer subject to carbon catabolite repression. This suggests that carbon catabolite repression of alkane degradation is regulated differently in Pseudomonas and in E. coli strains. These results also indicate that PalkBFGHJKL, the Palk promoter, might be useful in attaining high expression levels of heterologous genes in E. coli grown on inexpensive carbon sources which normally trigger carbon catabolite repression of native expression systems in this host.
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