ArticleJournal of bacteriology1999
The Escherichia coli Ada protein can interact with two distinct determinants in the sigma70 subunit of RNA polymerase according to promoter architecture: identification of the target of Ada activation at the alkA promoter.
Article in Journal of bacteriology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 55 citations in OpenAlex.
- The Versatile Attributes of MGMT: Its Repair Mechanism, Crosstalk with Other DNA Repair Pathways, and Its Role in Cancer.Cancers · 2024Review
- Different requirements for σ Region 4 in BvgA activation of the Bordetella pertussis promoters P(fim3) and P(fhaB).Journal of molecular biology · 2011Article
- Transcriptional control in the prereplicative phase of T4 development.Virology journal · 2010Review
- Protein-protein interactions between sigma(70) region 4 of RNA polymerase and Escherichia coli SoxS, a transcription activator that functions by the prerecruitment mechanism: evidence for "off-DNA" and "on-DNA" interactions.Journal of molecular biology · 2010Article
- A basic/hydrophobic cleft of the T4 activator MotA interacts with the C-terminus of E.coli sigma70 to activate middle gene transcription.Molecular microbiology · 2008Article
- Roles of effectors in XylS-dependent transcription activation: intramolecular domain derepression and DNA binding.Journal of bacteriology · 2008Article
- Linker regions of the RhaS and RhaR proteins.Journal of bacteriology · 2007Article
- Mutational analysis of sigma70 region 4 needed for appropriation by the bacteriophage T4 transcription factors AsiA and MotA.Journal of molecular biology · 2006Article
- Direct reversal of DNA alkylation damage.Chemical reviews · 2006Review
- Bacterial transcriptional regulators for degradation pathways of aromatic compounds.Microbiology and molecular biology reviews : MMBR · 2004Review
- Amino acid contacts between sigma 70 domain 4 and the transcription activators RhaS and RhaR.Journal of bacteriology · 2004Article
- Properties of Bacillus subtilis sigma A factors with region 1.1 and the conserved Arg-103 at the N terminus of region 1.2 deleted.Journal of bacteriology · 2004Article
- Identification of alkA gene related to virulence of Shigella flexneri 2a by mutational analysis.World journal of gastroenterology · 2003Article
- An intersubunit contact stimulating transcription initiation by E coli RNA polymerase: interaction of the alpha C-terminal domain and sigma region 4.Genes & development · 2003Article
- Growing repertoire of AraC/XylS activators.Journal of bacteriology · 2002Review
- Mapping of the Rsd contact site on the sigma 70 subunit of Escherichia coli RNA polymerase.Journal of bacteriology · 2001Article
- Regulatory responses of the adaptive response to alkylation damage: a simple regulon with complex regulatory features.Journal of bacteriology · 2000Article
- Transcription activation by a variety of AraC/XylS family activators does not depend on the class II-specific activation determinant in the N-terminal domain of the RNA polymerase alpha subunit.Journal of bacteriology · 2000Article
- Mechanism for a transcriptional activator that works at the isomerization step.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Recognition of overlapping nucleotides by AraC and the sigma subunit of RNA polymerase.Journal of bacteriology · 2000Article
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2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The methylated form of the Ada protein (meAda) activates transcription from the Escherichia coli ada, aidB, and alkA promoters with different mechanisms. In this study we identify amino acid substitutions in region 4 of the RNA polymerase subunit sigma70 that affect Ada-activated transcription at alkA. Substitution to alanine of residues K593, K597, and R603 in sigma70 region 4 results in decreased Ada-dependent binding of RNA polymerase to the alkA promoter in vitro and impairs alkA transcription both in vivo and in vitro, suggesting that these residues define a determinant for meAda-sigma70 interaction. In a previous study (P. Landini, J. A. Bown, M. R. Volkert, and S. J. W. Busby, J. Biol. Chem. 273:13307-13312, 1998), we showed that a set of negatively charged amino acids in sigma70 region 4 is involved in meAda-sigma70 interaction at the ada and aidB promoters. However, the alanine substitutions of positively charged residues K593, K597, and R603 do not affect meAda-dependent transcription at ada and aidB. Unlike the sigma70 amino acids involved in the interaction with meAda at the ada and aidB promoters, K593, K597, and R603 are not conserved in sigmaS, an alternative sigma subunit of RNA polymerase mainly expressed during the stationary phase of growth. While meAda is able to promote transcription by the sigmaS form of RNA polymerase (EsigmaS) at ada and aidB, it fails to do so at alkA. We propose that meAda can activate transcription at different promoters by contacting distinct determinants in sigma70 region 4 in a manner dependent on the location of the Ada binding site.
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