ArticleBiophysical journal1999
Diffusion barriers limit the effect of mobile calcium buffers on exocytosis of large dense cored vesicles.
Article in Biophysical journal, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 44 citations in OpenAlex.
- Close agreement between deterministic versus stochastic modeling of first-passage time to vesicle fusion.Biophysical journal · 2022Article
- Influence of T-Bar on Calcium Concentration Impacting Release Probability.Frontiers in computational neuroscience · 2022Article
- A study of the mechanisms of excitation-contraction coupling in frog skeletal muscle based on measurements of [CaJournal of muscle research and cell motility · 2018Review
- Resident Calmodulin Primes NMDA Receptors for CaBiophysical journal · 2017Article
- Cardiac BIN1 folds T-tubule membrane, controlling ion flux and limiting arrhythmia.Nature medicine · 2014Article
- Estimation of presynaptic calcium currents and endogenous calcium buffers at the frog neuromuscular junction with two different calcium fluorescent dyes.Frontiers in synaptic neuroscience · 2014Article
- Calcium transport and local pool regulate polycystin-2 (TRPP2) function in human syncytiotrophoblast.Biophysical journal · 2013Article
- Nanodomain Ca²⁺ of Ca²⁺ channels detected by a tethered genetically encoded Ca²⁺ sensor.Nature communications · 2012Article
- Ca2+ current versus Ca2+ channel cooperativity of exocytosis.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2009Article
- Otoferlin is critical for a highly sensitive and linear calcium-dependent exocytosis at vestibular hair cell ribbon synapses.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2009Article
- Modeling of quantal neurotransmitter release kinetics in the presence of fixed and mobile calcium buffers.Journal of computational neuroscience · 2008Article
- Determining calcium concentration in heterogeneous model systems using multiple indicators.Cell calcium · 2007Article
- High speed two-photon imaging of calcium dynamics in dendritic spines: consequences for spine calcium kinetics and buffer capacity.PloS one · 2007Article
- Synaptic transmission at retinal ribbon synapses.Progress in retinal and eye research · 2005Review
- Consequences of molecular-level Ca2+ channel and synaptic vesicle colocalization for the Ca2+ microdomain and neurotransmitter exocytosis: a monte carlo study.Biophysical journal · 2004Article
- A highly Ca2+-sensitive pool of vesicles contributes to linearity at the rod photoreceptor ribbon synapse.Neuron · 2004Article
- Recruitment of Ca(2+) release channels by calcium-induced Ca(2+) release does not appear to occur in isolated Ca(2+) release sites in frog skeletal muscle.The Journal of physiology · 2002Article
- New and corrected simulations of synaptic facilitation.Biophysical journal · 2002Article
- Extra activation component of calcium release in frog muscle fibres.The Journal of physiology · 2002Article
- Modeling study of exocytosis in neuroendocrine cells: influence of the geometrical parameters.Biophysical journal · 2000Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fast exocytosis in melanotropic cells, activated by calcium entry through voltage-gated calcium channels, is very sensitive to mobile calcium buffers (complete block at 800 microM ethylene glycol bis(beta-aminoethyl ether)-N,N,N'N'-tetraacetic acid (EGTA)). This indicates that calcium diffuses a substantial distance from the channel to the vesicle. Surprisingly, 1, 2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid (BAPTA), having a similar KD for calcium as EGTA but a approximately 100 times faster binding rate, blocked exocytosis only twice as effectively as EGTA. Using computer simulations, we demonstrate that this result cannot be explained by free diffusion and buffer binding rates. We hypothesized that local saturation of calcium buffers is involved. A diffusion barrier for both calcium and buffer molecules, located 50-300 nm from the membrane and reducing diffusion 1000 to 10,000 times, generated similar calcium concentrations for specific concentrations of EGTA and BAPTA. With such barriers, calcium rise phase kinetics upon short step depolarizations (2-20 ms) were faster for EGTA than for BAPTA, implying that short depolarizations should allow exocytosis with 50 microM EGTA but not with 25 microM BAPTA. This prediction was confirmed experimentally with capacitance measurements. Coupling exocytosis to calcium dynamics in the model, we found that a barrier with a approximately 3000 times reduced diffusion at approximately 130 nm beneath the membrane best explains the experimentally observed effects of EGTA and BAPTA on block and kinetics of release.
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