ArticleThe Journal of cell biology1999
A monoclonal antibody to the COOH-terminal acidic portion of Ran inhibits both the recycling of Ran and nuclear protein import in living cells.
Article in The Journal of cell biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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Who cites it
24 citing papers in PubMed, 63 citations in OpenAlex.
- RANBP1 (RAN Binding Protein 1): The Missing Genetic Piece in Cancer Pathophysiology and Other Complex Diseases.Cancers · 2023Review
- The complex relationship of Wnt-signaling pathways and cilia.Current topics in developmental biology · 2023Article
- Inhibition of Kpnβ1 mediated nuclear import enhances cisplatin chemosensitivity in cervical cancer.BMC cancer · 2021Article
- Y-box protein-associated acidic protein (YBAP1/C1QBP) affects the localization and cytoplasmic functions of YB-1.Scientific reports · 2018Article
- Differences in the nuclear export mechanism between myocardin and myocardin-related transcription factor A.The Journal of biological chemistry · 2013Article
- Directionality of nucleocytoplasmic transport of the retroviral gag protein depends on sequential binding of karyopherins and viral RNA.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- The mitotic arrest deficient protein MAD2B interacts with the small GTPase RAN throughout the cell cycle.PloS one · 2009Article
- Localized regulation of axonal RanGTPase controls retrograde injury signaling in peripheral nerve.Neuron · 2008Article
- Importin-beta and the small guanosine triphosphatase Ran mediate chromosome loading of the human chromokinesin Kid.The Journal of cell biology · 2008Article
- The mechanism of nuclear export of Smad3 involves exportin 4 and Ran.Molecular and cellular biology · 2006Article
- Cross-talk between snurportin1 subdomains.Molecular biology of the cell · 2005Article
- A multifunctional domain in human CRM1 (exportin 1) mediates RanBP3 binding and multimerization of human T-cell leukemia virus type 1 Rex protein.Molecular and cellular biology · 2003Article
- The carboxy-terminal region of the human immunodeficiency virus type 1 protein Rev has multiple roles in mediating CRM1-related Rev functions.Journal of virology · 2002Article
- Activation of mitochondrial voltage-dependent anion channel by apro-apoptotic BH3-only protein Bim.Oncogene · 2002Article
- Constitutive and IFN-gamma-induced nuclear import of STAT1 proceed through independent pathways.The EMBO journal · 2002Article
- Transport into and out of the nucleus.Microbiology and molecular biology reviews : MMBR · 2001Review
- Rat CRM1 is responsible for the poor activity of human T-cell leukemia virus type 1 Rex protein in rat cells.Journal of virology · 2001Article
- Transforming growth factor-beta induces nuclear import of Smad3 in an importin-beta1 and Ran-dependent manner.Molecular biology of the cell · 2001Article
- Dimerization of sterol regulatory element-binding protein 2 via the helix-loop-helix-leucine zipper domain is a prerequisite for its nuclear localization mediated by importin beta.Molecular and cellular biology · 2001Article
- The nuclear export receptor Xpo1p forms distinct complexes with NES transport substrates and the yeast Ran binding protein 1 (Yrb1p).Molecular biology of the cell · 2001Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
A small GTPase Ran is a key regulator for active nuclear transport. In immunoblotting analysis, a monoclonal antibody against recombinant human Ran, designated ARAN1, was found to recognize an epitope in the COOH-terminal domain of Ran. In a solution binding assay, ARAN1 recognized Ran when complexed with importin beta, transportin, and CAS, but not the Ran-GTP or the Ran-GDP alone, indicating that the COOH-terminal domain of Ran is exposed via its interaction with importin beta-related proteins. In addition, ARAN1 suppressed the binding of RanBP1 to the Ran-importin beta complex. When injected into the nucleus of BHK cells, ARAN1 was rapidly exported to the cytoplasm, indicating that the Ran-importin beta-related protein complex is exported as a complex from the nucleus to the cytoplasm in living cells. Moreover, ARAN1, when injected into the cultured cells induces the accumulation of endogenous Ran in the cytoplasm and prevents the nuclear import of SV-40 T-antigen nuclear localization signal substrates. From these findings, we propose that the binding of RanBP1 to the Ran-importin beta complex is required for the dissociation of the complex in the cytoplasm and that the released Ran is recycled to the nucleus, which is essential for the nuclear protein transport.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.