ArticleInfection and immunity1999
Cloning and expression of the dnaK gene of Campylobacter jejuni and antigenicity of heat shock protein 70.
Article in Infection and immunity, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
12 citing papers in PubMed, 34 citations in OpenAlex.
- Transcriptome analysis of aerotolerant and aerosensitiveFrontiers in microbiology · 2025Article
- Cooperative Regulation ofMicroorganisms · 2020Article
- Identification of temperature regulated factors ofAIMS microbiology · 2017Article
- Antimicrobial and Virulence-Modulating Effects of Clove Essential Oil on the Foodborne Pathogen Campylobacter jejuni.Applied and environmental microbiology · 2016Article
- Modification of intestinal microbiota and its consequences for innate immune response in the pathogenesis of campylobacteriosis.Clinical & developmental immunology · 2013Review
- Comparative proteomic analyses of Streptococcus suis serotype 2 cell wall-associated proteins.Current microbiology · 2011Article
- Cj0596 is a periplasmic peptidyl prolyl cis-trans isomerase involved in Campylobacter jejuni motility, invasion, and colonization.BMC microbiology · 2009Article
- A temperature-regulated Campylobacter jejuni gluconate dehydrogenase is involved in respiration-dependent energy conservation and chicken colonization.Molecular microbiology · 2008Article
- The HtrA protease of Campylobacter jejuni is required for heat and oxygen tolerance and for optimal interaction with human epithelial cells.Applied and environmental microbiology · 2005Article
- Use of genome-wide expression profiling and mutagenesis to study the intestinal lifestyle of Campylobacter jejuni.Infection and immunity · 2005Article
- Gene expression profile of Campylobacter jejuni in response to growth temperature variation.Journal of bacteriology · 2003Article
- Transcriptional analysis of major heat shock genes of Helicobacter pylori.Journal of bacteriology · 2000Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Campylobacter jejuni is a leading cause of infectious diarrhea throughout the world. In addition, there is growing evidence that Guillain-Barré syndrome, an inflammatory demyelinating disease of the peripheral nervous system, is frequently preceded by C. jejuni infection. In the present study, the hrcA-grpE-dnaK gene cluster of C. jejuni was cloned and sequenced. The dnaK gene consists of an open reading frame of 1,869 bp and encodes a protein with a high degree of homology to other bacterial 70-kDa heat shock proteins (HSPs). The overall percentages of identity to the HSP70 proteins of Helicobacter pylori, Borrelia burgdorferi, Chlamydia trachomatis, and Bacillus subtilis were calculated to be 78.1, 60.5, 57.2, and 53. 8%, respectively. Regions similar to the Escherichia coli sigma70 promoter consensus sequence and to a cis-acting regulatory element (CIRCE) are located upstream of the hrcA gene. Following heat shock, a rapid increase of dnaK mRNA was detectable, which reached its maximum after 20 to 30 min. A 6-His-tagged recombinant DnaK protein (rCjDnaK-His) was generated in E. coli, after cloning of the dnaK coding region into pET-22b(+), and purified by affinity and gel filtration chromatography. Antibody responses to rCjDnaK-His were significantly elevated, compared to those of healthy individuals, in about one-third of the serum specimens obtained from C. jejuni enteritis patients.
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