ArticleInfection and immunity1999
A 55-kilodalton immunodominant antigen of Porphyromonas gingivalis W50 has arisen via horizontal gene transfer.
Article in Infection and immunity, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 69 citations in OpenAlex.
- Exploring the Genetic and Functional Diversity ofInternational journal of molecular sciences · 2025Article
- Metagenome-derived SusD-homologs affiliated with Bacteroidota bind to synthetic polymers.Applied and environmental microbiology · 2024Article
- Prevalence and Phylogenetic Analysis of Lipoprotein-GeneAntibiotics (Basel, Switzerland) · 2023Article
- The RagA and RagB proteins of Porphyromonas gingivalis.Molecular oral microbiology · 2021Review
- Oral dysbiosis induced byJournal of oral microbiology · 2020Article
- Novel large-scale chromosomal transfer in Bacteroides fragilis contributes to its pan-genome and rapid environmental adaptation.Microbial genomics · 2017Article
- Article
- Structure of RagB, a major immunodominant outer-membrane surface receptor antigen of Porphyromonas gingivalis.Molecular oral microbiology · 2016Article
- Porphyromonas gingivalis RagB is a proinflammatory signal transducer and activator of transcription 4 agonist.Molecular oral microbiology · 2015Article
- Article
- Prevalence of Porphyromonas gingivalis four rag locus genotypes in patients of orthodontic gingivitis and periodontitis.PloS one · 2013Article
- Comprehensive transcriptome analysis of the periodontopathogenic bacterium Porphyromonas gingivalis W83.Journal of bacteriology · 2012Article
- Selective sorting of cargo proteins into bacterial membrane vesicles.The Journal of biological chemistry · 2011Article
- The core genome of the anaerobic oral pathogenic bacterium Porphyromonas gingivalis.BMC microbiology · 2010Article
- The rag locus of Porphyromonas gingivalis might arise from Bacteroides via horizontal gene transfer.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2010Article
- Novel features of the polysaccharide-digesting gliding bacterium Flavobacterium johnsoniae as revealed by genome sequence analysis.Applied and environmental microbiology · 2009Article
- Entry of Porphyromonas gingivalis outer membrane vesicles into epithelial cells causes cellular functional impairment.Infection and immunity · 2009Article
- Genome sequence of the cellulolytic gliding bacterium Cytophaga hutchinsonii.Applied and environmental microbiology · 2007Article
- The rag locus of Porphyromonas gingivalis contributes to virulence in a murine model of soft tissue destruction.Infection and immunity · 2007Article
- Sequence diversity and antigenic variation at the rag locus of Porphyromonas gingivalis.Infection and immunity · 2005Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A 55-kDa outer membrane protein of Porphyromonas gingivalis W50 is a significant target of the serum immunoglobulin G antibody response of periodontal disease patients and hence may play an important role in host-bacterium interactions in periodontal disease. The gene encoding the 55-kDa antigen (ragB, for receptor antigen B) was isolated on a 9.5-kb partial Sau3AI fragment of P. gingivalis W50 chromosomal DNA in pUC18 by immunoscreening with a monoclonal antibody to this antigen. The 1.6-kb open reading frame (ORF) encoding RagB was located via subcloning and nested-deletion analysis. Sequence analysis demonstrated the presence of an upstream 3.1-kb ORF (ragA) which is cotranscribed with ragB. A number of genetic characteristics suggest that the ragAB locus was acquired by a horizontal gene transfer event. These include a significantly reduced G+C content relative to that of the P. gingivalis chromosome (42 versus 48%) and the presence of mobility elements flanking this locus in P. gingivalis W50. Furthermore, Southern blotting and PCR analyses showed a restricted distribution of this locus in laboratory and clinical isolates of this bacterium. The association of ragAB+ P. gingivalis with clinical status was examined by PCR analysis of subgingival samples. ragAB+ was not detected in P. gingivalis-positive shallow pockets from periodontal disease patients but was present in 36% of the P. gingivalis-positive samples from deep pockets. These data suggest that the ragAB locus was acquired by certain P. gingivalis strains via horizontal gene transfer and that the acquisition of this locus may facilitate the survival of these strains at sites of periodontal destruction.
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