ArticleInfection and immunity1999
Role of DnaK in in vitro and in vivo expression of virulence factors of Vibrio cholerae.
Article in Infection and immunity, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 45 citations in OpenAlex.
- Impacts of feeding preweaned calves milk containing drug residues on the functional profile of the fecal microbiota.Scientific reports · 2018Trial
- Intestinal colonization againstAIMS microbiology · 2023Review
- Pathogenicity and virulence regulation ofVirulence · 2020Review
- Sequence Determinants Spanning -10 Motif and Spacer Region Implicated in UniqueFrontiers in microbiology · 2019Article
- Phylogeny ofFrontiers in microbiology · 2017Article
- Novel strategy for biofilm inhibition by using small molecules targeting molecular chaperone DnaK.Antimicrobial agents and chemotherapy · 2015Article
- Reduced virulence of the Vibrio cholerae fadD mutant is due to induction of the extracytoplasmic stress response.Infection and immunity · 2013Article
- Bile salt-induced intermolecular disulfide bond formation activates Vibrio cholerae virulence.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- A fadD mutant of Vibrio cholerae is impaired in the production of virulence factors and membrane localization of the virulence regulatory protein TcpP.Infection and immunity · 2011Article
- Modulation of adherence, invasion, and tumor necrosis factor alpha secretion during the early stages of infection by Streptococcus pneumoniae ClpL.Infection and immunity · 2007Article
- Global gene expression and phenotypic analysis of a Vibrio cholerae rpoH deletion mutant.Journal of bacteriology · 2007Article
- Differential proteomic analysis of the Bacillus anthracis secretome: distinct plasmid and chromosome CO2-dependent cross talk mechanisms modulate extracellular proteolytic activities.Journal of bacteriology · 2006Article
- Role of the histone-like nucleoid structuring protein in colonization, motility, and bile-dependent repression of virulence gene expression in Vibrio cholerae.Infection and immunity · 2006Article
- Bile acids induce cholera toxin expression in Vibrio cholerae in a ToxT-independent manner.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Effect of bile on the cell surface permeability barrier and efflux system of Vibrio cholerae.Journal of bacteriology · 2004Article
- Effect of anaerobiosis on expression of virulence factors in Vibrio cholerae.Infection and immunity · 2004Article
- The global regulator ArcA modulates expression of virulence factors in Vibrio cholerae.Infection and immunity · 2003Article
- Genomic scale analysis of Pasteurella multocida gene expression during growth within the natural chicken host.Infection and immunity · 2002Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The dnaK gene of Vibrio cholerae was cloned, sequenced, and used to construct a dnaK insertion mutant which was then used to examine the role of DnaK in expression of the major virulence factors of this important human pathogen. The central regulator of several virulence genes of V. cholerae is ToxR, a transmembrane DNA binding protein. The V. cholerae dnaK mutant grown in standard laboratory medium exhibited phenotypes characteristic of cells deficient in ToxR activity. Using Northern blot analysis and toxR transcriptional fusions, we demonstrated a reduction in expression of the toxR gene in the dnaK mutant strain together with a concomitant increase in expression of a htpG-like heat shock gene that is located immediately upstream and is divergently transcribed from toxR. This may be due to increased heat shock induction in the dnaK mutant. In vivo, however, although expression from heat shock promoters in the dnaK mutant was similar to that observed in vitro, expression of both toxR and htpG was comparable to that by the parental strain. In both strains, in vivo expression of toxR was significantly higher than that observed in vitro, but no reciprocal decrease in htpG expression was observed. These results suggest that the modulation of toxR expression in vivo may be different from that observed in vitro.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.