ArticleThe Biochemical journal1999
The endosome fusion regulator early-endosomal autoantigen 1 (EEA1) is a dimer.
Article in The Biochemical journal, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
What it found
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Who cites it
37 citing papers in PubMed, 125 citations in OpenAlex.
- Rab21 recruits EEA1 and competes with Rab5 for Rabex-5 activation.Frontiers in cell and developmental biology · 2025Article
- Deterministic early endosomal maturations emerge from a stochastic trigger-and-convert mechanism.Nature communications · 2023Article
- Membrane-binding mechanism of the EEA1 FYVE domain revealed by multi-scale molecular dynamics simulations.PLoS computational biology · 2021Article
- Polyphosphoinositide-Binding Domains: Insights from Peripheral Membrane and Lipid-Transfer Proteins.Advances in experimental medicine and biology · 2019Review
- Conserved salt-bridge competition triggered by phosphorylation regulates the protein interactome.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Rabies virus co-localizes with early (Rab5) and late (Rab7) endosomal proteins in neuronal and SH-SY5Y cells.Virologica Sinica · 2017Article
- A modification switch on a molecular switch: Phosphoregulation of Rab7 during endosome maturation.Small GTPases · 2016Article
- Membrane tethering.F1000prime reports · 2014Review
- Phosphoinositides and vesicular membrane traffic.Biochimica et biophysica acta · 2012Review
- The p97 ATPase associates with EEA1 to regulate the size of early endosomes.Cell research · 2012Article
- A tethering complex dimer catalyzes trans-SNARE complex formation in intracellular membrane fusion.Bioarchitecture · 2012Article
- Molecular analysis of protein-phosphoinositide interactions.Current topics in microbiology and immunology · 2012Review
- Role of Rab GTPases in membrane traffic and cell physiology.Physiological reviews · 2011Review
- Article
- Defining the role of a FYVE domain in the localization and activity of a cAMP phosphodiesterase implicated in osmoregulation in Trypanosoma cruzi.Molecular microbiology · 2011Article
- The Sir Hans Krebs Lecture. How a lipid mediates tumour suppression. Delivered on 29 June 2010 at the 35th FEBS Congress in Gothenburg, Sweden.The FEBS journal · 2010Article
- Identification and structural characterization of FYVE domain-containing proteins of Arabidopsis thaliana.BMC plant biology · 2010Article
- Translation of the phosphoinositide code by PI effectors.Nature chemical biology · 2010Review
- Structural basis for Rab GTPase recognition and endosome tethering by the C2H2 zinc finger of Early Endosomal Autoantigen 1 (EEA1).Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Rab GTPases as coordinators of vesicle traffic.Nature reviews. Molecular cell biology · 2009Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
EEA1, an early-endosomal protein originally identified as an autoantigen, is essential for endocytic membrane fusion. It interacts with early endosomes via binding to the membrane lipid phosphatidylinositol 3-phosphate (PtdIns3P) and the active form of the small GTPase Rab5. Most of the EEA1 sequence contains heptad repeats characteristic of proteins involved in coiled-coil protein-protein interactions. Here we have investigated the ability of EEA1 to self-interact. Crosslinking of cytosolic and recombinant EEA1 resulted in the disappearance of the 180-kDa monomer in SDS/PAGE and the strong appearance of a approximately 350-kDa crosslinked product. Glycerol gradient centrifugation experiments indicated that native EEA1 had the same hydrodynamic properties as the approximately 350-kDa crosslinked complex. Two-hybrid analysis indicated that N- and C-terminal fragments of EEA1 can interact with themselves, but not with each other, suggesting that EEA1 forms parallel coiled-coil dimers. The ability of the C-terminus of EEA1 to dimerize correlates with its ability to bind to Rab5 and early endosomes, whereas its binding to PtdIns3P is independent of dimerization. These data enable us to propose a model for the quaternary structure of EEA1.
Indexed as
Identifiers
10024533PMC1220083W2025536670What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.