ArticleRNA (New York, N.Y.)1999
The stability and fate of a spliced intron from vertebrate cells.
Article in RNA (New York, N.Y.), 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
36 citing papers in PubMed.
- DBR1 orchestrates the fate of lariat RNA: debranching-dependent turnover and function.Nucleic acids research · 2025Review
- Mirtrons in Human Cancers.Onco · 2025Article
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- Intron turnover is essential to the development and pathogenicity of the plant pathogenic fungus Fusarium graminearum.Communications biology · 2022Article
- Impact of scaffolding protein TNRC6 paralogs on gene expression and splicing.RNA (New York, N.Y.) · 2021Article
- Restriction of an intron size en route to endothermy.Nucleic acids research · 2021Article
- Two classes of active transcription sites and their roles in developmental regulation.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Adult-Onset Myopathy with Constitutive Activation of Akt following the Loss of hnRNP-U.iScience · 2020Article
- Elongin C Contributes to RNA Polymerase II Degradation by the Interferon Antagonist NSs of La Crosse Orthobunyavirus.Journal of virology · 2020Article
- Molecular functions and specific roles of circRNAs in the cardiovascular system.Non-coding RNA research · 2018Review
- Molecular roles and function of circular RNAs in eukaryotic cells.Cellular and molecular life sciences : CMLS · 2018Review
- Guide RNA engineering for versatile Cas9 functionality.Nucleic acids research · 2016Review
- Article
- Stable intronic sequence RNAs (sisRNAs): a new layer of gene regulation.Cellular and molecular life sciences : CMLS · 2016Review
- Minor Contribution of Chimeric Host-HIV Readthrough Transcripts to the Level of HIV Cell-Associated gag RNA.Journal of virology · 2016Article
- Stable intronic sequence RNAs have possible regulatory roles in Drosophila melanogaster.The Journal of cell biology · 2015Article
- Lariat intronic RNAs in the cytoplasm of Xenopus tropicalis oocytes.RNA (New York, N.Y.) · 2014Article
- Multiplexed and programmable regulation of gene networks with an integrated RNA and CRISPR/Cas toolkit in human cells.Molecular cell · 2014Article
- Lariat sequencing in a unicellular yeast identifies regulated alternative splicing of exons that are evolutionarily conserved with humans.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Article
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Authors and funding
4 authors.
Funding
Abstract
Introns constitute most of the length of typical pre-mRNAs in vertebrate cells. Thus, the turnover rate of introns may significantly influence the availability of ribonucleotides and splicing factors for further rounds of transcription and RNA splicing, respectively. Given the importance of intron turnover, it is surprising that there have been no reports on the half-life of introns from higher eukaryotic cells. Here, we determined the stability of IVS1Cbeta1, the first intron from the constant region of the mouse T-cell receptor-beta, (TCR-beta) gene. Using a tetracycline (tet)-regulated promoter, we demonstrate that spliced IVS1Cbeta1 and its pre-mRNA had half-lives of 6.0+/-1.4 min and 3.7+/-1.0 min, respectively. We also examined the half-lives of these transcripts by using actinomycin D (Act.D). Act.D significantly stabilized IVS1Cbeta1 and its pre-mRNA, suggesting that Act.D not only blocks transcription but exerts rapid and direct posttranscriptional effects in the nucleus. We observed that in vivo spliced IVS1Cbeta1 accumulated predominantly as lariat molecules that use a consensus branchpoint nucleotide. The accumulation of IVS1Cbeta1 as a lariat did not result from an intrinsic inability to be debranched, as it could be debranched in vitro, albeit somewhat less efficiently than an adenovirus intron. Subcellular-fractionation and sucrose-gradient analyses showed that most spliced IVS1Cbeta1 lariats cofractionated with pre-mRNA, but not always with mRNA in the nucleus. Some IVS1Cbeta1 also appeared to be selectively exported to the cytoplasm, whereas TCR-beta pre-mRNA remained in the nucleus. This study constitutes the first detailed analysis of the stability and fate of a spliced nuclear intron in vivo.
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Registered trials
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