ArticleMolecular and cellular biology1999
Hsp60 is targeted to a cryptic mitochondrion-derived organelle ("crypton") in the microaerophilic protozoan parasite Entamoeba histolytica.
Article in Molecular and cellular biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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Who cites it
50 citing papers in PubMed, 178 citations in OpenAlex.
- Phylogenetic ancestry of Metamonada proteins points to a common origin of mitochondria in all eukaryotes.Molecular biology and evolution · 2026Article
- The history of entamoebiasis.Parasitology · 2025Review
- A Mitosome With Distinct Metabolism in the Uncultured Protist Parasite Paramikrocytos canceri (Rhizaria, Ascetosporea).Genome biology and evolution · 2023Article
- The mitosome of the anaerobic parasitic protist Entamoeba histolytica: A peculiar and minimalist mitochondrion-related organelle.The Journal of eukaryotic microbiology · 2022Review
- Evidence for an Independent Hydrogenosome-to-Mitosome Transition in the CL3 Lineage of Fornicates.Frontiers in microbiology · 2022Article
- Inheritance of the reduced mitochondria of Giardia intestinalis is coupled to the flagellar maturation cycle.BMC biology · 2021Article
- Energy metabolism in anaerobic eukaryotes and Earth's late oxygenation.Free radical biology & medicine · 2019Review
- Idiosyncratic Genome Degradation in a Bacterial Endosymbiont of Periodical Cicadas.Current biology : CB · 2017Article
- The Physiology of Phagocytosis in the Context of Mitochondrial Origin.Microbiology and molecular biology reviews : MMBR · 2017Review
- An Interactome-Centered Protein Discovery Approach Reveals Novel Components Involved in Mitosome Function and Homeostasis in Giardia lamblia.PLoS pathogens · 2016Article
- Potential Interaction of Plasmodium falciparum Hsp60 and Calpain.The Korean journal of parasitology · 2015Article
- Evidence that the Entamoeba histolytica Mitochondrial Carrier Family Links Mitosomal and Cytosolic Pathways through Exchange of 3'-Phosphoadenosine 5'-Phosphosulfate and ATP.Eukaryotic cell · 2015Article
- Gene similarity networks provide tools for understanding eukaryote origins and evolution.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Mitochondrial evolution.Cold Spring Harbor perspectives in biology · 2012Review
- Biochemistry and evolution of anaerobic energy metabolism in eukaryotes.Microbiology and molecular biology reviews : MMBR · 2012Review
- Characterization of microRNAs from Orientobilharzia turkestanicum, a neglected blood fluke of human and animal health significance.PloS one · 2012Article
- The Trichomonas vaginalis hydrogenosome proteome is highly reduced relative to mitochondria, yet complex compared with mitosomes.International journal for parasitology · 2011Article
- Mitosomes in trophozoites and cysts of the reptilian parasite Entamoeba invadens.Eukaryotic cell · 2011Article
- Localization and targeting of an unusual pyridine nucleotide transhydrogenase in Entamoeba histolytica.Eukaryotic cell · 2010Article
- The essentials of protein import in the degenerate mitochondrion of Entamoeba histolytica.PLoS pathogens · 2010Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Entamoeba histolytica is a microaerophilic protozoan parasite in which neither mitochondria nor mitochondrion-derived organelles have been previously observed. Recently, a segment of an E. histolytica gene was identified that encoded a protein similar to the mitochondrial 60-kDa heat shock protein (Hsp60 or chaperonin 60), which refolds nuclear-encoded proteins after passage through organellar membranes. The possible function and localization of the amebic Hsp60 were explored here. Like Hsp60 of mitochondria, amebic Hsp60 RNA and protein were both strongly induced by incubating parasites at 42 degreesC. 5' and 3' rapid amplifications of cDNA ends were used to obtain the entire E. histolytica hsp60 coding region, which predicted a 536-amino-acid Hsp60. The E. histolytica hsp60 gene protected from heat shock Escherichia coli groEL mutants, demonstrating the chaperonin function of the amebic Hsp60. The E. histolytica Hsp60, which lacked characteristic carboxy-terminal Gly-Met repeats, had a 21-amino-acid amino-terminal, organelle-targeting presequence that was cleaved in vivo. This presequence was necessary to target Hsp60 to one (and occasionally two or three) short, cylindrical organelle(s). In contrast, amebic alcohol dehydrogenase 1 and ferredoxin, which are bacteria-like enzymes, were diffusely distributed throughout the cytosol. We suggest that the Hsp60-associated, mitochondrion-derived organelle identified here be named "crypton," as its structure was previously hidden and its function is still cryptic.
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