ArticleMolecular and cellular biology1999
Nrg1 is a transcriptional repressor for glucose repression of STA1 gene expression in Saccharomyces cerevisiae.
Article in Molecular and cellular biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
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55 citing papers in PubMed, 111 citations in OpenAlex.
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- New Aspects of Invasive Growth Regulation Identified by Functional Profiling of MAPK Pathway Targets inGenetics · 2020Article
- Misactivation of multiple starvation responses in yeast by loss of tRNA modifications.Nucleic acids research · 2020Article
- Regulated repression governs the cell fate promoter controlling yeast meiosis.Nature communications · 2020Article
- Cyc8p and Tup1p transcription regulators antagonistically regulate Flo11p expression and complexity of yeast colony biofilms.PLoS genetics · 2018Article
- QTL mapping of volatile compound production in Saccharomyces cerevisiae during alcoholic fermentation.BMC genomics · 2018Article
- Determinants of selection in yeast evolved by genome shuffling.Biotechnology for biofuels · 2018Article
- Overexpression of smORF YNR034W-A/EGO4 in Saccharomyces cerevisiae increases the fermentative efficiency of Agave tequilana Weber must.Journal of industrial microbiology & biotechnology · 2017Article
- Modulation of Circadian Gene Expression and Metabolic Compensation by the RCO-1 Corepressor of Neurospora crassa.Genetics · 2016Article
- De-repression of CSP-1 activates adaptive responses to antifungal azoles.Scientific reports · 2016Article
- Microevolution of Candida albicans in macrophages restores filamentation in a nonfilamentous mutant.PLoS genetics · 2014Article
- Coupling mRNA synthesis and decay.Molecular and cellular biology · 2014Review
- Discrimination between thermodynamic models of cis-regulation using transcription factor occupancy data.Nucleic acids research · 2014Article
- Comparative evolution of morphological regulatory functions in Candida species.Eukaryotic cell · 2013Article
- Nitrogen transporter and assimilation genes exhibit developmental stage-selective expression in maize (Zea mays L.) associated with distinct cis-acting promoter motifs.Plant signaling & behavior · 2013Article
- Crystal structure of the N-terminal domain of the yeast general corepressor Tup1p and its functional implications.The Journal of biological chemistry · 2012Article
- Shields up: the Tup1-Cyc8 repressor complex blocks coactivator recruitment.Genes & development · 2011Article
- The stress response factors Yap6, Cin5, Phd1, and Skn7 direct targeting of the conserved co-repressor Tup1-Ssn6 in S. cerevisiae.PloS one · 2011Article
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Authors and funding
5 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Expression of genes encoding starch-degrading enzymes is regulated by glucose repression in the yeast Saccharomyces cerevisiae. We have identified a transcriptional repressor, Nrg1, in a genetic screen designed to reveal negative factors involved in the expression of STA1, which encodes a glucoamylase. The NRG1 gene encodes a 25-kDa C2H2 zinc finger protein which specifically binds to two regions in the upstream activation sequence of the STA1 gene, as judged by gel retardation and DNase I footprinting analyses. Disruption of the NRG1 gene causes a fivefold increase in the level of the STA1 transcript in the presence of glucose. The expression of NRG1 itself is inhibited in the absence of glucose. DNA-bound LexA-Nrg1 represses transcription of a target gene 10.7-fold in a glucose-dependent manner, and this repression is abolished in both ssn6 and tup1 mutants. Two-hybrid and glutathione S-transferase pull-down experiments show an interaction of Nrg1 with Ssn6 both in vivo and in vitro. These findings indicate that Nrg1 acts as a DNA-binding repressor and mediates glucose repression of the STA1 gene expression by recruiting the Ssn6-Tup1 complex.
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