ArticleMolecular and cellular biology1999
C/EBPalpha regulates generation of C/EBPbeta isoforms through activation of specific proteolytic cleavage.
Article in Molecular and cellular biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 108 citations in OpenAlex.
- The Role of CYP3A in Health and Disease.Biomedicines · 2022Review
- The "Janus" Role of C/EBPs Family Members in Cancer Progression.International journal of molecular sciences · 2020Review
- Article
- C/EBPß Isoform Specific Gene Regulation: It's a Lot more Complicated than you Think!Journal of mammary gland biology and neoplasia · 2020Review
- Analysis of the interplay between all-trans retinoic acid and histone deacetylase inhibitors in leukemic cells.Archives of toxicology · 2017Article
- TSC1 controls IL-1β expression in macrophages via mTORC1-dependent C/EBPβ pathway.Cellular & molecular immunology · 2016Article
- Suppression of the pregnane X receptor during endoplasmic reticulum stress is achieved by down-regulating hepatocyte nuclear factor-4α and up-regulating liver-enriched inhibitory protein.Toxicological sciences : an official journal of the Society of Toxicology · 2015Article
- Article
- CCAAT/enhancer binding protein β in relation to ER stress, inflammation, and metabolic disturbances.BioMed research international · 2015Review
- Stable conditional expression and effect of C/ebpβ-LIP in adipocytes using the pSLIK system.Journal of molecular endocrinology · 2013Article
- Expression of an entire bacterial operon in plants.Plant physiology · 2012Article
- Article
- Calreticulin is a negative regulator of bronchial smooth muscle cell proliferation.Journal of allergy · 2012Article
- Pro-inflammatory gene expression and neurotoxic effects of activated microglia are attenuated by absence of CCAAT/enhancer binding protein β.Journal of neuroinflammation · 2011Article
- The orphan nuclear receptor RORalpha restrains adipocyte differentiation through a reduction of C/EBPbeta activity and perilipin gene expression.Molecular endocrinology (Baltimore, Md.) · 2009Article
- CCAAT/enhancer-binding protein beta: its role in breast cancer and associations with receptor tyrosine kinases.Expert reviews in molecular medicine · 2009Review
- CCAAT/enhancer-binding proteins and the pathogenesis of retrovirus infection.Future microbiology · 2009Review
- Article
- Differentiation-induced cleavage of Cutl1/CDP generates a novel dominant-negative isoform that regulates mammary gene expression.Molecular and cellular biology · 2006Article
- All-trans retinoic acid down-regulates human albumin gene expression through the induction of C/EBPbeta-LIP.The Biochemical journal · 2006Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
C/EBPalpha and C/EBPbeta are intronless genes that can produce several N-terminally truncated isoforms through the process of alternative translation initiation at downstream AUG codons. C/EBPbeta has been reported to produce four isoforms: full-length 38-kDa C/EBPbeta, 35-kDa LAP (liver-enriched transcriptional activator protein), 21-kDa LIP (liver-enriched transcriptional inhibitory protein), and a 14-kDa isoform. In this report, we investigated the mechanisms by which C/EBPbeta isoforms are generated in the liver and in cultured cells. Using an in vitro translation system, we found that LIP can be generated by two mechanisms: alternative translation and a novel mechanism-specific proteolytic cleavage of full-length C/EBPbeta. Studies of mice in which the C/EBPalpha gene had been deleted (C/EBPalpha-/-) showed that the regulation of C/EBPbeta proteolysis is dependent on C/EBPalpha. The induction of C/EBPalpha in cultured cells leads to induced cleavage of C/EBPbeta to generate the LIP isoform. We characterized the cleavage activity in mouse liver extracts and found that the proteolytic cleavage activity is specific to prenatal and newborn livers, is sensitive to chymostatin, and is completely abolished in C/EBPalpha-/- animals. The lack of cleavage activity in the livers of C/EBPalpha-/- mice correlates with the decreased levels of LIP in the livers of these animals. Analysis of LIP production during liver regeneration showed that, in this system, the transient induction of LIP is dependent on the third AUG codon and most likely involves translational control. We propose that there are two mechanisms by which C/EBPbeta isoforms might be generated in the liver and in cultured cells: one that is determined by translation and a second that involves C/EBPalpha-dependent, specific proteolytic cleavage of full-length C/EBPbeta. The latter mechanism implicates C/EBPalpha in the regulation of posttranslational generation of the dominant negative C/EBPbeta isoform, LIP.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.